HPLC法测定人血浆中华法林药物浓度

目的：建立HPLC法测定血浆中华法林浓度方法，并用此方法监测本院术后患者华法林血浓度。方法：采用ZORBAXEclipseXDB-C8色谱柱（150mm×4.6mm,5μm），流动相：0.1%醋酸：甲醇（35∶65），流速：1.0ml·min-1；检测波长：308nm；柱温：30℃；进样量：20μl。二极管阵列检测器检测。结果：线性范围为0.1～5.12μg·ml-1，提取回收率为85.0%～102.3%，RSD为1.2%～3.9%（n=6）。日内、日间RSD均小于5%，重复性好。结论：该方法准确、简便、精密度高，可用于华法林血药浓度监测。

Determination of Warfarin in Human Plasma by HPLC

Objective： To establish a simple HPLC method for the determination of warfarin in human plasma and analyze the results of 100 monitoring reports. Methods： Warfarin was extracted from the plasma samples by acetonitrile. The extract was separated on a ZORBAX Eclipse XDB-C8 column （150 mm×4.6 mm, 5 μm）with a mobile phase consisting of methanol and 0.1% acetic acid （65∶35） at flow rate of 1.0 ml·min-1 and detected by a diode array detector. The detection wavelength was at 308nm and the sample size was 20 μl. Results： The calibration curve was linear within the range of 0.1-5.12μg·ml-1. The average recovery and RSD was ranged from 85.0% to 102.3% and 1.2% to 3.9%（n=6）, respectively. Inter- and intra-day RSD were 1.1%-1.8% and 1.6%-2.9%, respectively.Conclusion： The proposed method is rapid, convenient and accurate in the detection of warfarinin concentration in plasma.