HPLC法测定聚甲酚磺醛阴道栓的含量

目的：建立HPLC法测定聚甲酚磺醛阴道栓中聚甲酚磺醛含量的方法。方法：采用PhenomexexLuna-C18色谱柱（150mm×4.6mm，5μm），以0.5%四甲基氢氧化铵溶液（用磷酸调节pH至4.0）-甲醇（30∶70）为流动相；流速为1.0ml·min-1；检测波长为280nm；柱温为35℃，进样量为20μl。结果：聚甲酚磺醛在10～160g·ml-1范围内峰面积与浓度呈良好的线性关系（r=0.9999）；平均加样回收率为99.35%，RSD=0.92%（n=9）。结论：本方法操作简便，结果准确，可用于该制剂中聚甲酚磺醛的含量测定。

Determination of Policerseilen Vaginal Suppositories by HPLC

Objective： To establish an HPLC method for the determination of policerseilen in policerseilen vaginal suppositories. Methods： A Phenomexex Luna-C18 column（150 mm×4.6 mm，5 μm） was used, the mobile phase consisted of 0.5% tetramethyl ammonium hydroxide solution （adjusting pH to 4.0 with phosphoric acid） and methanol （30∶70）. The flow rate was 1.0 ml·min-1, the detection wavelength was 280nm, the column temperature was 35℃ and the injection volume was 20 μl. Results：Policerseilen had a good linearity within the range of 10 160 g·ml-1（r=0.999 9）, the average recovery was 99.35% and RSD was 0.92%（n=9）. Conclusion：The established method is accurate, sensitive and simple. It can be applied in the determination of policerseilen vaginal suppositories.