桂产藿香蓟乙醇提取物抗炎作用及其机制研究

目的：探讨桂产藿香蓟乙醇提取物的抗炎作用及其作用机制。方法：采用二甲苯诱导小鼠耳廓肿胀，观察桂产藿香蓟乙醇提取物对二甲苯诱导小鼠耳廓肿胀的影响；采用角叉菜胶致小鼠足肿胀法，观察桂产藿香蓟乙醇提取物对角叉菜胶致小鼠足肿胀的影响，并分别测定小鼠炎性组织中丙二醛（MDA）、前列腺素E2（PGE2）和超氧化物歧化酶（SOD）的活性；采用角叉菜胶致大鼠足肿胀法，观察桂产藿香蓟乙醇提取物对角叉菜胶诱导大鼠足肿胀的影响，同时测定大鼠血清肿瘤坏死因子（TNF-α）、白介素-1β（IL-1β）和白介素-6（IL-6）的含量。结果：与空白对照组比较，桂产藿香蓟乙醇提取物能显著抑制小鼠耳廓肿胀及足肿胀（P〈0.05或0.01），其中高、中、低剂量组（6.0,3.0,1.5g·kg-1）耳廓肿胀和足肿胀抑制率分别为29.24%，16.42%，11.21%和28.66%，18.79%，13.13%，并能降低小鼠炎足中炎性组织PGE2、MDA含量，提高其SOD活力（P〈0.05或0.01）；显著抑制大鼠的足肿胀（P〈0.05或0.01），其中在3h时高、中、低剂量组（6.0,3.0,1.5g·kg-1）的足肿胀抑制率分别为43.69%，36.01%，23.29%，并能显著降低肿胀足大鼠血清TNF-α、IL-1β和IL-6的含量（P〈0.05或0.01）。结论：桂产藿香蓟乙醇提取物抗炎作用显著，其机制可能与清除氧自由基、减少炎症因子和致炎细胞因子的释放有关。

Anti-inflammatory Effect and Underlying Mechanism of Ethanol Extracts of Ageratum Conyzoides. L. in Guangxi

Objective： To explore the anti inflammatory effects and the underlying mechanisms of ethanol extract of Ageratum conyzoides. L. from Guangxi. Methods： The auricle edema model was induced by dimethylbenzene in the mice and the paw edema model was induced by carrageenan respectively in the mice and rats to study the anti inflammatory effects of ethanol extract of Ageratum conyzoides. L. from Guangxi. The content of malondiadehyde （MDA） and proateglandin E2 （PGE2）, and the activity of superoxide dismutase（SOD） in the mouse edema paw was measured. The contents of tumour necrosisfactor α （TNF-α）, interleukin 1β （IL-1β） and interleukin 6（IL-6） in the rat serum were detected as well. Results：Compared with the model control group, the ethanol extracts of Ageratum conyzoides. L. from Guangxi could remarkably inhibit auricle edema in the mice and paw edema in the mice and rats（P〈0.05 or P〈0.01）, the inhibition ratio for high, medium and low dosage group（6.0,3.0,1.5g·kg-1）was29.24%，16.42% and 11.21% in the auricle edema mice and 28.66%，18.79% and 13.13% in the paw edema mice , respectively. It could remarkably reduce MDA and PGE2 content and enhance the activity of SOD in the mouse inflammatory tissue（P〈0.05 or P〈0.01）. In the paw edema rats, the inhibition ratio for high, medium and low dosage group（4.5,2.3, 1.2 g·kg-1）was 43.69%, 36.01% and 23.29% at the 3rd h, respectively , and it also could remarkably reduce serum TNF-α, IL-1β and IL-6 content（P〈0.05 or P〈0.01）.Conclusion：The ethanol extracts of Ageratum conyzoides. L. from Guangxi show significantly anti inflammatory effects, and the mechanisms may be related to the ability of scavenging oxygen free radicals and reducing the release of inflammatory cytokines and proinflammatory cytokines.