| HPLC法测定染料木素丙烯酸树脂纳米粒的药物含量及包封率 |
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| 引用本文： | 徐娜,纪宏宇,唐景玲,吴琳华.HPLC法测定染料木素丙烯酸树脂纳米粒的药物含量及包封率[J].中国药师,2014(2):204-206. |
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| 作者姓名： | 徐娜 纪宏宇 唐景玲 吴琳华 |
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| 作者单位： | 哈尔滨医科大学附属第四医院药学部；哈尔滨 150086;哈尔滨医科大学附属第二医院药学部，黑龙江省高校重点实验室；;哈尔滨医科大学附属第二医院药学部，黑龙江省高校重点实验室；哈尔滨医科大学药学院;哈尔滨医科大学附属第二医院药学部，黑龙江省高校重点实验室；哈尔滨医科大学药学院 |
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| 基金项目： | 国家自然科学基金项目（面上项目，重点项目，重大项目），黑龙江省青年科学基金项目 |
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| 摘 要： | 目的：建立测定丙烯酸树脂纳米粒中染料木素含量的HPLC方法。方法：色谱柱：DiamonsilTM C18柱（250mm×4．6mm，5μm）；流动相：甲醇-水=85：15（v：v）；流速：1．0ml·min-1；柱温：25℃；检测波长：261nm；进样量：10斗l、结果：药物峰与辅料峰分离度良好，染料木素浓度在1．0～100．0μg·ml-1范围内线性关系良好（r=0．9998），平均回收率为99．85％（RSD=0．65％，n=9）。结论：本方法简便，快捷，准确度高，专属性强，适用于测定染料木素丙烯酸树脂纳米粒的含量及包封率。
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| 关 键 词： | 染料木素 丙烯酸树脂 纳米粒 高效液相色谱 含量 包封率 |
| 收稿时间： | 2013/10/17 0:00:00 |
| 修稿时间： | 2013/11/30 0:00:00 |
Determination of Content and Encapsulation Efficiency of Genistein loaded Eudragit Nanoparticles by HPLC |
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| Xu N,Jing Hongyu,Tang Jingling and Wu Linhua.Determination of Content and Encapsulation Efficiency of Genistein loaded Eudragit Nanoparticles by HPLC[J].China Pharmacist,2014(2):204-206. |
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| Authors: | Xu N Jing Hongyu Tang Jingling and Wu Linhua |
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| Institution: | Department of Pharmacy, the Fourth Affiliated Hospital of Harbin Medical University, Key Laboratory of College in Heilongjiang Province; Harbin 150086, China;Department of Pharmacy, the Second Affiliated Hospital of Harbin Medical University;;Department of Pharmacy, the Second Affiliated Hospital of Harbin Medical University;College of Pharmacy, Harbin Medical University, Harbin 150086, China;Department of Pharmacy, the Second Affiliated Hospital of Harbin Medical University;College of Pharmacy, Harbin Medical University, Harbin 150086, China |
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| Abstract: | Objective： To establish an HPLC method for the determination of genistein in genistein-loaded Eudragit nanoparticles. Methods： The HPLC analysis was carried out on a DiamonsilTM C18 （250mm×4.6mm,5μm）column with the mobile phase of methanol-water （85： 15） at the flow rate of 1.0 ml ·min-1. The detection wavelength was set at 261nm and the column temperature was 25℃. Results： A good separation was displayed between the drug peak and the excipient peaks. An excellent linearity range for genistein was obtained from 1.0 μg·ml-1 to 100.0 μg·ml-1 （ r = 0.999 8 ）. The recovery of genistein was 99.85% （ RSD = 0.65% ,n = 9）. Conclusion： The method is simple, rapid, accurate and specific, which is suitable for the determination of content and encapsulation efficiency of genistein-loaded nanoparticles. |
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| Keywords: | Genistein Eudragit Nanoparticles HPLC Content Encapsulation efficiency |
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