羊膜对氧氟沙星滴眼液兔角膜通透性的影响

目的探讨羊膜对氧氟沙星滴眼液兔角膜通透性的影响。方法先给108只新西兰大白兔进行样本编号,再按号码单纯随机抽样并分为6组,每组18只,分别为正常角膜组、正常角膜羊膜移植组、机械刮除角膜上皮组、机械刮除上皮联合羊膜移植组、角膜碱烧伤组、角膜碱烧伤联合羊膜移植组。0．3％氧氟沙星眼液滴眼,每15min滴眼1次,共4次,最后1次滴眼后5、30min,1、2、4、6h,于每个时间点每组18只大白兔中任意处死3只兔抽取房水。高效液相法检测房水中氧氟沙星的浓度。结果刮除角膜上皮组与正常角膜上皮组比较,药物的前房浓度均明显增高（均P〈0．05）。正常角膜羊膜移植组5、30min、1h房水中氧氟沙星浓度与正常角膜组无明显差异（均P〉0．05）,而2、4、6h则明显高于正常角膜组（均P〈0．05）;机械刮除上皮联合羊膜移植组2、4、6h房水中氧氟沙星浓度亦明显高于机械刮除角膜上皮组（均P〈0．05）,而两组间5、30min、1h房水中氧氟沙星浓度比较无明显差异（均P〉0．05）;角膜碱烧伤联合羊膜移植组6h以内房水中氧氟沙星浓度均高于碱烧伤组（均P〈0．05）。结论羊膜具有一定药物蓄积和缓释作用,增加了氧氟沙星在角膜表面的停留时间,使药物的通透性增高。

The effects of amniotic membrane on corneal penetration of ofloxacin

Objective To evaluate the comeal penetration of ofloxacin following amniotic membrane transplantation (AMT). Methods One hundred and eight New Zealand rabbits were divided into 6 groups: mechanical remove of corneal epithelium with and without AMT; comeal alkali bum with and without AMT, and normal comea with and without AMT. Topical ofloxacin was administered four times at interval of every 15 minutes. Five min, 30 min, 1 h, 2 h, 4 h and 6 h after the last administration, 3 rabbits from each group were sacrificed and aqueous humor was collected. The concentration of ofloxacin was detected by high pressure liquid chromatography ( HPLC). Results The ability of ofloxacin to penetrating comea was improved obviously in de-epithelialized groups and cornea alkali burn groups no matter AMT was performed or not(P <0. 05). The concentration of ofloxacin in the aqueous humor in normal cornea with and without AMT showed no statistical difference within 1 h after administration. But at the last three time points, the ofloxacin concentration in AMT group was greater than that in the group without AMT ( P < 0. 05). The comparison between the de-epithelialized with and without AMT obtained the similar result At every tested time point, the ofloxacin concentration in aqueous humor of comeal alkali bum with AMT group was statistically greater than that of the group without AMT ( P < 0. 05). Conclusion Amniotic membrane could store and release the drug slowly, results in the increase of contact time between ofloxacin and comeal surface and promotes the corneal penetration of ofloxacin.