Evidence for endogenous reovirus-like particles in a tissue culture cell line from Drosophila melanogaster |
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| Authors: | R Haars H Zentgraf E Gateff F A Bautz |
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| Institution: | 1. Institut für Molekulare Genetik, Universität Heidelberg, 6900 Heidelberg, West Germany;2. Deutsches Krebsforschungzentrum, 6900 Heidelberg, West Germany;3. Biologisches Institut I, Universität Freiburg, 7800 Freiburg, West Germany |
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| Abstract: | Virus-like particles were isolated from the Blood IIIBlood III cell line of Drosophila melanogaster by sucrose and cesium chloride density centrifugation. The particles have a buoyant density of 1.37 g/cm3 in cesium chloride and a diameter of 568 Å The viral genome contains double-stranded RNA by the criteria of resistance to ribonuclease A and nuclease S1. Polyacrylamide gel electrophoresis resolves the RNA into 10-segments and an additional minor component rangina in molecular weight from 0.55 × 106 to 2.75 × 106. |
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| Keywords: | To whom reprint requests should be addressed |
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