| 消化道肿瘤的脉管内侵犯与微转移、微血管密度关系的研究 |
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| 引用本文： | 范文华,吴佩,王彦东. 消化道肿瘤的脉管内侵犯与微转移、微血管密度关系的研究[J]. 安徽医药, 2009, 13(6): 649-651 |
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| 作者姓名： | 范文华 吴佩 王彦东 |
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| 作者单位： | 安徽省芜湖市第二人民医院普外科;皖南医学院附属弋矶山医院普外科,安徽,芜湖,241001 |
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| 摘 要： | 目的研究胃肠癌微转移与微血管密度的特点,分析肿瘤的脉管内侵犯与微转移、微血管密度之间的关系。方法采用HE染色和免疫组化染色判断肿瘤的脉管内侵犯;采用RT-PCR技术,检测46例胃癌、大肠癌患者门静脉血中CK20 mRNA的表达;采用免疫组化方法,检测46例患者的微血管密度。结果46例患者存在脉管内侵犯的17例中,12例CK20 mRNA阳性,5例阴性。29例非脉管内侵犯的患者中,9例CK20 mRNA阳性,20例阴性。脉管内侵犯的微转移阳性率高于无脉管内侵犯的阳性率（P〈0.05）;脉管内侵犯的平均微血管密度为（29.2±3.3）,高于非脉管内侵犯的平均微血管密度25.4±4.7（P〈0.05）;免疫组化染色发现脉管内侵犯17例,优于HE染色发现脉管内侵犯的9例（P〈0.05）。结论用分子生物学的方法证实了肿瘤对血管的侵犯与微转移、微血管密度之间的关系密切,可能是影响患者预后的因素之一;免疫组化染色在检测脉管内侵犯方面优于HE染色。
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| 关 键 词： | 脉管内侵犯 RT-PCR 微血管密度 微转移 |
microvessel density and micrometastasis in patients with gastric cancer and colorectal cancer with vascular invasion |
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| FAN Wen-hua,WU Pei,WANG Yan-dong. microvessel density and micrometastasis in patients with gastric cancer and colorectal cancer with vascular invasion[J]. Anhui Medical and Pharmaceutical Journal, 2009, 13(6): 649-651 |
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| Authors: | FAN Wen-hua WU Pei WANG Yan-dong |
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| Affiliation: | FAN Wen-hua1,WU Pei2,WANG Yan-dong1(1.Department of General Surgery,WuHu Second People's Hospital,Wuhu 241001,2.Department of General Surgery,Yijishan Hospital of Wannan Medical College,Wuhu 241001) |
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| Abstract: | Aim Vascular invasion is one of the most important clinicopathologic characteristics of malignant tumor.Several researchers have implied the prognostic value of vascular invasion for various types of cancer.This study was conducted to evaluate correlation between vascular invasion and microvessel density of tissue and metastasis in blood.Methods Vascular invasion was detected by both Hematoxylin-eosin staining and immunohistochemical staining.Blood samples from 17 patients with vascular invasion and 29 pati... |
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| Keywords: | RT-PCR |
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