放免法测定胰岛素C-肽时脂血标本的预处理及其临床意义

为了消除脂血对测定结果的影响,探讨胰岛素(INS)、C-肽(C-P)放免法测定时脂血标本的预处理方法及意义.将每份待测标本(包括INS质控血清、正常人血清及高脂血清)分为两份,一份直接测定,另一份用95g/L聚乙二醇(PEG)处理标本后,再以放免法测定INS、C-P.测定结果进行t检验及相关性分析.标本经PEG处理离心后脂质被沉淀,质控血清及正常人血清INS、C-P测定值与未处理者无统计学差异(P》0.1).高脂血清与未处理标本测定值比较显著降低(P《0.001).因此正常情况下,PEG处理标本不影响INS、C-P的测定值;标本经PEG处理后,去除了高脂、INS抗体、INS原,从而消除对测定结果的干扰,提高了结果的准确性.

Pretreated High Lipid Serum Method and Its Significance before Determine INS C-P by RIA

The method of predigest high lipid serum before determine INS and C-P for elimination of the high lipid in serum was studied.The specimen was divided into two groups, one was directly tested, the other was pretreated, then determined(including INS and QC of serum, normal and high lipid serum, pretreating serum).The results were compared by examine and analysed correlativity.Results show that INS and C-P of the specimens pretreated by PEG,were no statistic difference in relation to direct tested (P>0.05) and those were close correlativity(P<0.001)in normal.The results of high lipid serum after the specimens were predigest by PEG were marked by decrease in relation to direct tested(P<0.001).High lipid and INS antibody were eliminated by PEG so that was increased the results nicety.