全自动生化分析仪测定高密度脂蛋白胆固醇后对镁检测的交叉污染

目的探讨全自动生化分析仪测定高密度脂蛋白胆固醇（HDL-C）后对镁（Mg）检测的交叉污染。方法单独检测甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL—C）、高密度脂蛋白胆固醇（HDL—C）4种试剂中的Mg浓度。检测组分为TG、TC、LDL—C、HDL—C4组。TG检测组：先测160份TG，紧接着测160份Mg；TC、LDL—C、HDL—C3组的测定过程同TG检测组。设置比色杯特殊清洗程序后，先测160份HDL—C后紧接着测160份Mg。结果HDL—C检测组Mg检测结果显著高于对照组（P〈0．01）。其余3组与对照组比较差异无统计学意义（P〉0．05）。通过设置特殊清洗程序后，HDL—C检测组Mg检测结果与对照组相比差异无统计学意义（P〉0．05）。结论全自动生化分析仪测定HDL—C后对Mg测定有严重的交叉污染。可以通过设置比色杯特殊清洗程序消除HDL—C测定对Mg测定的干扰。

The cross-contamination that high density lipoprotein cholesterol affected the detection of magnesium by automatic chemistry analyzer

Objective To study the cross-contamination that high-density lipoprotein cholesterol （HDL-C） affected the detection of magnesium （Mg） by automatic chemistry analyzer. Methods The concentration of Mg was analyzed by automatic chemistry analyzer in total glyeeride （TG）, total cholesterol （TC）, low-density lipoprotein cholesterol （LDL-C） and HDL-C groups. Mg was tested immediately after HDL-C been analyzed, and then compared to the control group which were analyzed alone. The protocol of TG, TC, LDL-C groups was as above. By setting the special washing program, the item HDL-C was executed primarily and then the item Mg was analyzed. Results The value of Mg in the HDL-C group raised highly than the control group （ P 〈 0.01 ）. The other groups had no significant difference （ P 〉 0.05 ）. After setting the special washing program, there was no significant difference between the HDL-C group and the control group （ P 〉 0.05 ）. Conclusions HDL-C affected the detection of magnesium. The cross-contamination can be eliminated by resetting the special washing program in the automatic chemistry analyzer.