| 大孔吸附树脂纯化葛花总异黄酮工艺研究 |
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| 引用本文： | 杨向东,程靖,刘博,陈四艳,史自东,徐海南. 大孔吸附树脂纯化葛花总异黄酮工艺研究[J]. 中医药导报, 2008, 14(11): 70-72 |
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| 作者姓名： | 杨向东 程靖 刘博 陈四艳 史自东 徐海南 |
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| 作者单位： | 1. 湖北丽益医药科技有限公司,湖北,武汉,430061
2. 华中师范大学,湖北,武汉,430079 |
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| 摘 要： | 目的:筛选分离葛花总异黄酮的最佳树脂,并对影响分离的各种因素进行系统的研究,使分离工艺达到最优化.方?浇 法:研究7种不同类型吸附树脂对葛花总异黄酮的吸附特性,用紫外分光光度法测定葛花总异黄酮的含量对工艺进行评价.结果:AB-8型大孔树脂对葛花总异黄酮的吸附性能较好,其吸附纯化条件为葛花提取物上样浓度为3.0 mg·mL-1,吸附流速为2BV·h(树脂床体积),以60%乙醇作为洗脱剂进行洗脱效果最佳.经AB-8处理后的葛花总异黄酮含量可达50%以上.结论:该法简单可行,分离效果好,能满足于大生产的要求.
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| 关 键 词： | 大孔吸附树脂 葛花总异黄酮 分离纯化 |
Study on Separation and Purification of the Total Isoflavones from F los Puerariae With Macroporotls Adsorption Resin |
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| YANG Xiang-dong,CHENG Jing,LIU Bo,CHEN Si-yan,SHI zi-dong,XU Hai-nan. Study on Separation and Purification of the Total Isoflavones from F los Puerariae With Macroporotls Adsorption Resin[J]. GUIDING JOURNAL OF TCM, 2008, 14(11): 70-72 |
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| Authors: | YANG Xiang-dong CHENG Jing LIU Bo CHEN Si-yan SHI zi-dong XU Hai-nan |
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| Affiliation: | YANG Xiang-dong1,CHENG Jing2,LIU Bo1,CHEN Si-yan1,SHI Zi-dong1,XU Hai-nan1 1.Hubei Liyi Medicine , Pharmacy Technology Co.Ltd.,Wuhan,China,430061,2.Central China Normal University,430079 |
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| Abstract: | Objective:To screen the optimal separation of the total isoflavones from F los Puerariae by selecting appropriate macroporous resins and to study systematically the factors which affect separation.Methods:To study 7 kinds of macroporous resins for isolation and purification of the total isofavones from F los Puerariae,and evaluated the separaring efficiency by measuring the concentration of total isofavones in F los Puerariae with UV spectrophotometer.Results:The AB-8 macroporous resin had the best separati... |
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| Keywords: | Macroporous adsorption resin total isoflavones of F los Puerariae separation and purificatio |
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