细胞周期蛋白E和肿瘤转移抑制基因nm23-H1与鼻咽癌

目的 探讨细胞周期蛋白E(cyclin E)和肿瘤转移抑制基因nm23-H1在鼻咽癌组织中的表达及其临床意义.方法 用免疫组化SP法检测42例鼻咽癌和28例正常鼻黏膜上皮的病理组织切片标本中cyclin E和nm23-H1蛋白的表达.结果 cyclin E蛋白在鼻咽癌组织中的阳性表达率为59.5%(25/42),显著高于对照组中的17.9%(5/28);nm23-H1蛋白在鼻咽癌组织中的阳性表达率为42.8%(18/42),显著低于对照组中的100%(28/28);cyclin E蛋白在低分化组和有淋巴结转移组中的阳性表达率分别为74.2%和77.8%,明显高于高、中分化组和无淋巴结转移组中的18.1%和26.7%;nm23-H1蛋白在低分化组和有淋巴结转移组中阳性表达率分别为25.8%和25.9%,明显低于高、中分化组和无淋巴结转移组中的90.9%和73.3%;cyclin E和nm23-H1在鼻咽癌组织中的表达呈负相关.结论 cyclin E过表达和nm23-H1表达下调在鼻咽癌的发生、发展和转移中起重要作用.

Expression and its significance of cycline E and nm23-H1 in nasopharyngeal carcinoma

OBJECTIVE To examine the expression of cycline E and tumor suppressing gene nm23-H1 in nasopharyngeal carcinoma(NPC). METHODS The expression of cycline E and nm23-H1 in 48 specimens of NPC tissue and 28 specimens of normal nasopharyngeal tissue was examined by immunohistochemistry. RESULTS The expression rate of cycline E was significantly higher in tumor tissues than that in normal tissues (59.5 % vs 17.9 %). The expression rate of nm23-H1 was significantly lower in tumor tissues than that in normal tissues (42.8 % vs 100 %). The expression rate of cycline E was significantly higher in poorly differentiated tumor tissues and tumor tissues with lymphatic metastasis (74.2 % and 77.8 %) than that in moderately differentiated and well-differentiated tumor tissues and tumor tissues without lymphatic metastasis (18.1 % and 26.7 %). The expression rate of nm23-H1 was significantly lower in poorly differentiated tumor tissues and tumor tissues with lymphatic metastasis (25.8 % and 25.9 %)than that in moderately differentiated and well-differentiated tumor tissues and tumor tissues without lymphatic metastasis (90.9 % and 73.3 %). The expression of cycline E and nm23-H1 in NPC tissues had negative correlation. CONCLUSION The high level expression of cycline E and the low level expression of nm23-H1 protein may be associated with the development and metastasis of NPC.