托马斯液复合应用康斯特保护液对大鼠离体心肌保护作用的研究

目的：观察托马斯液（STH）复合应用不同剂量康斯特保护液（HTK）对大鼠长时间缺血心肌的保护作用。方法：雌性Wistar大鼠40只，体重230～280g，随机分为S、H、S＋H10、S＋H20和S＋H30共5组（n=8）。麻醉后开胸取出心脏立即连于langendorff灌注装置，制备心脏缺血再灌注模型，S组用STH液15ml／kg每30min灌注1次。H组用HTK液30ml／kg单次灌注，其余3组均先用STH液15ml／kg灌注后分别续灌HTK液10ml／kg、20ml／kg、30ml／kg。分别留取停搏前、再灌注15、30min时冠脉流出液2ml用于检测心肌乳酸脱氢酶（LDH）、肌酸激酶（CK）。实验末留取心尖部心肌组织，用于检测心肌组织三磷酸腺苷（ATP）、丙二醛（MDA）、超氧化物歧化酶（SOD）含量。结果：与S组比较，其余4组复灌15、30min后冠状动脉流出液心肌酶CK、LDH含量较低（P〈0．05，P〈0．01）；与S、H、S＋H10各组相比，复灌30min后，S＋H20组及S＋H30组心肌ATP、SOD含量增高（P〈0．05，P〈0．01），心肌MDA含量降低（P〈0．05，P〈0．01）。结论：STH液复合一定剂量的HTK液诱导并维持心脏停搏能增加大鼠离体心肌ATP、SOD含量，降低MDA含量．减轻缺血再灌注损伤。

Myocardial protection effects of STH solution combined with HTK solution on isolated rat hearts

Objective： To observe myocardial protection effects of STH solution combined with HTK solution on isolated rat hearts after long time ischemia. Methods： 40 female Wistar rats, weight 230-280 g, were randomized as 5 groups ： group S; group H; group S ＋H10; group S ＋H20 and group S ＋H30. Langendorff ischemic-reperfusion model was developed by isolated rat after anesthesia, group S and group H was perfused with STH solution 15 ml/kg every 30 minutes or HTK solution 30 ml/kg once time, in the other 3 groups, STH solution was perfused as 15 ml/kg at first, then perfused with HTK solution 10 ml/kg, 20 ml/kg,30 ml/kg respectively. 2 ml of coronary flow was taken before the cardiac arrest and after reperfused 15 min, 30 min to detect myocardial leak enzyme LDH, CK. Tissue at heart apexes was retented after experiment. ATP, MDA and SOD contents in myocardium were detected. Results：Compare with group S, the content of CK and LDH was lower in the other 4 groups （P〈0.05,P〈0.01）; after reperfused 30 min, the content of ATP, SOD was increased significantly in group S＋H20 and S＋H30, yet the content of MDA was decreased （P〈0.05,P〈0.01）. Conclusion： STH solution combined with certain dosage of HTK solution to induce and maintain the cardiac arrest can increase the content of ATP,SOD ,decrease the content of MDA in vitro rat myocardium and mitigate the ischemic-reperfusion injure.