骨科疾病中蛋白质组学的应用进展

背景：蛋白质组学是后基因组时代的新研究领域，它以组织或细胞的全部蛋白质结构与功能为研究对象，通过检测蛋白质来分析各种疾病的重要病理生理变化。目的：就目前蛋白质组学相关技术在骨科各种疾病中的应用与展望作一综述。方法：以“组织构建/蛋白质组学/骨科学/椎间盘退变/骨质疏松/骨性关节炎/成骨细胞/破骨细胞/综述”为中文检索词，以“proteomics/orthopedics/review/osteoblasts metabolism/osteoclasts metabolism/ intervertebraldisc degeneration/osteoporosis/osteoarthritis/serum related orthopedic diseases”为英文检索词，检索珠江三角洲数字图书馆联盟期刊全文数据库和Pubmed外文数据库2001年1月至2013年9月有关蛋白质组学在骨科各类疾病当中的基础和临床研究的相关文献。纳入有关报告蛋白质组学在椎间盘病变、骨质疏松症及骨性关节炎等方面的应用的文献。排除重复性研究和不典型报道。结果与结论：蛋白质组学已成功应用于成骨细胞、破骨细胞代谢，椎间盘退变、骨质疏松症、骨性关节炎及骨科相关疾病的血清等方面，以上所提及的疾病均为各类人群的常见多发病，这些相关的基础临床研究无疑为人类健康带来了巨大的福音，相信蛋白质组学相关技术在基础医学和临床医学中会取得更大的突破，将展示广阔的发展前景。中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程全文链接：

Proteomics and its application in orthopedic diseases

BACKGROUND: Proteomics is a new field of research in the post-genomic era. The research subjects of proteomics include the structure and function of entire proteins in tissues or cells. It analyses the vital changes of pathological and physiological process of diseases by examining proteins.OBJECTIVE: To summarize the application of proteomics in orthopedic diseases and its prospect.METHODS: Articles about the application of proteomics in orthopedic diseases and its prospect were retrieved from the Digital Library Alliance of Zhujiang River Delta and PubMed with the key words of “proteomics, orthopedics, review, osteoblasts metabolism, osteoclasts metabolism, intervertebral disc degeneration, osteoporosis, osteoarthritis, serum related orthopedic diseases” in both Chinese and English from January 2001 to September 2013. Reproductive research and atypical reports were excluded. RESULTS AND CONCLUSION: Proteomics has been applied in the osteoblasts metabolism, osteoclasts metabolism, intervertebral disc degeneration, osteoporosis, osteoarthritis and serum related orthopedic diseases that are the most common illnesses in all types of people. These basic and clinical studies undoubtedly bring great benefit for human health, and proteomics technology will get a bigger breakthrough in basic medicine and clinical medicine, and it shows us a broad prospect.