miR-183-5p/mTOR信号轴介导有氧糖酵解促进 胃癌细胞增殖

目的 探讨miR-183-5p/mTOR信号轴是否通过影响胃癌细胞有氧糖酵解过程而促进胃癌细胞增殖，为胃癌的治疗提供新靶点。方法 使用转染试剂盒对MGC-803胃癌细胞进行inhibitor NC、miR-183-5p inhibitor、mimics NC、miR-183-5p mimics转染；葡萄糖、乳酸、腺嘌呤核苷三磷酸（adenosine triphosphate，ATP）检测试剂盒检测低葡萄糖、乳酸、ATP水平变化；通过蛋白质印迹和聚合酶链反应（polymerase chain reaction，PCR）检测雷帕霉素机械靶蛋白（mechanistic target of rapamycin，mTOR）mRNA、葡萄糖转运蛋白（glucose transporter 1，GLUT1）和乳酸脱氢酶（recombinant lactate dehydrogenase A，LDHA）蛋白；二苯基四氮唑溴盐法（multiple table tournament，MTT）检测MGC-803胃癌细胞活性。结果 与inhibitor NC组相比，miR-183-5p inhibitor在胃癌细胞中低表达（P<0.001），与mimics NC组相比，miR-183-5p inhibitor组在胃癌细胞中高表达miR-183-5p（P<0.0001）；过表达miR-183-5p可促进MGC-803胃癌细胞对葡萄糖的消耗（P<0.01），生成更多的乳酸和ATP（P<0.01）、上调LDHA和GLUT1蛋白（P<0.01）、促进mTOR mRNA表达、促进胃癌细胞增殖。结论 miR-183-5p/mTOR信号轴通过调控有氧糖酵解相关蛋白表达促进MGC-803胃癌细胞增殖。

miR-183-5p/mTOR signaling axis mediates the effect of aerobic glycolysis on the proliferation of gastric cancer cells

Objective To investigate whether miR-183-5p/mTOR signaling axis regulates the proliferation of gastric cancer cells by affecting the process of aerobic glycolysis, and to provide a new target for the treatment of gastric cancer. Methods MGC-803 gastric cancer cells were transfected with inhibitor NC, miR-183-5p inhibitor, mimics NC and miR-183-5p mimics using transfection kit. Glucose, lactic acid and ATP detection kits were used to detect the changes of low glucose, lactic acid and ATP levels. mTOR mRNA, GLUT1 and LDHA proteins were detected by WB and PCR. The activity of MGC-803 gastric cancer cells was detected by MTT assay. Results Compared with the inhibitor NC group, the expression of miR-183-5p inhibitor was down-regulated in gastric cancer cells (P<0.001). miR-183-5p inhibitor significantly increased the expression of miR-183-5p in gastric cancer cells (P<0.0001), indicated that the transfection was successful. Overexpression of miR-183-5p promoted glucose consumption of MGC-803 gastric cancer cells (P<0.01), produced more lactate and ATP (P<0.01), up-regulated LDHA and GLUT1 proteins (P<0.01), promoted mTOR mRNA expression, and promoted the proliferation of gastric cancer cells. Conclusion miR-183-5p /mTOR signaling axis affects the proliferation of MGC-803 gastric cancer cells by regulating the expression of aerobic glycolytic related proteins.