自固化磷酸钙人工骨Ⅱ修复兔腱-骨损伤的生物力学变化

背景：磷酸钙人工骨与重组人骨形态发生蛋白2在实验中均有成骨诱导作用，有修复腱骨界面损伤的可能。目的：评估自固化磷酸钙人工骨Ⅱ(含重组人骨形态发生蛋白2)修复兔肩袖腱-骨界面损伤后的生物力学变化。方法：取27只成年健康家兔，其中3只直接取双侧肩关节腱-骨界面标本作为正常组，余下的24只家兔双侧肩关节接受兔肩袖急性断裂腱-骨止点重建手术，术中实验组12只填塞自固化磷酸钙人工骨Ⅱ，对照组12只不填塞任何药物。分别于术后2，4，8周采集标本行生物力学测试。结果与结论：实验组各时间点肩袖腱-骨界面最大抗拉强度均高于对照组(P < 0.001)，但低于正常组(P < 0.001)；实验组术后8周肩袖腱-骨界面刚度高于对照组(P < 0.001)，但低于正常组(P < 0.001)。表明自固化磷酸钙人工骨Ⅱ可以在术后早期提高兔腱-骨界面的最大抗拉强度和刚度，增强腱-骨界面结合力，促进腱骨界面愈合。中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程全文链接：

Biomechanical changes of injured rabbit tendon-to-bone interface during repair with calcium phosphate cement-II

BACKGROUND: Calcium phosphate cement and recombinant human bone morphogenetic protein-2 both have bone osteoinductivity. Maybe both of them could promote repair of tendon-to-bone interface damage.OBJECTIVE:To evaluate the effect of calcium phosphate cement-II containing recombinant human bone morphogenetic protein-2 on tendon-to-bone interface healing after rotator cuff injury . METHODS:Twenty-seven adult healthy rabbits were enrolled in the study. The tendon-to-bone interface of the bilateral shoulder joints was taken from three rabbits as normal group, and the bilateral shoulder joints of the other 24 rabbits were subjected to acute rupture of the rotator cuff and tendon-to-bone reconstructive surgery as experimental group (n=12) and control group (n=12). Rabbits in the experimental group were treated with calcium phosphate cement-II, while those in the control group treated with nothing. Specimens were collected at 2, 4, 8 postoperative weeks for biomechanical test. RESULTS AND CONCLUSION: The maximum tensile strength of the rotator cuff in the experimental group was higher than that in the control group (P < 0.001), but lower than that in the normal group (P < 0.001). At postoperative 8 weeks, the rigidity of rotator cuff of the experiment group was higher than the control group (P < 0.001) but lower than the normal group (P < 0.001). These findings indicate that calcium phosphate cement-II can improve the maximum tensile strength and rigidity of rabbit tendon-to-bone interface in the early postoperative period, enhance tendon-to-bone interface binding force, and promote tendon-to-bone interface healing.