硅烷偶联剂对纯钛表面改性及细胞相容性的影响

背景：目前国内将硅烷偶联用于金属表面预处理的报道较少。 目的：对NaOH碱处理的钛片行硅烷化改性，观察硅烷膜的表面形貌与结构特征及细胞相容性。 方法：对制得的纯钛试件行NaOH碱处理，然后分别以8%，15%，33%浓度的KH-550硅烷偶联剂进行改性处理，以纯钛片和碱处理的钛片为对照组，采用扫描电镜观察改性处理钛片表面微观形貌，采用能谱仪分析改性处理钛片表面的成分。将纯钛片、碱处理的钛片及不同浓度KH-550硅烷偶联剂改性处理的钛片分别与犬骨髓间充质干细胞共培养，观察材料表面细胞的形态及黏附。 结果与结论：硅烷膜由许多呈脑浆状的小片构成，排列紧密，主要由C、N、O、Si等元素组成。当硅烷溶液浓度为8%时，钛表面难以形成较完整的硅烷膜；当硅烷溶液浓度为15%时，钛表面形成的硅烷膜表面较8%浓度组钛表面相对较完整，但可仍见有较多裂纹，难以形成致密的硅烷膜；当硅烷溶液浓度为33%时，纯钛表面能形成致密的硅烷膜。犬骨髓间充质干细胞在33%浓度硅烷膜处理后钛基体上的黏附情况明显优于纯钛、碱处理钛片及8%，15%浓度硅烷膜处理后的钛片。说明33%硅烷化改性纯钛表面硅烷膜较完整，并且具有良好的生物相容性，可促进骨髓间质干细胞在活性层表面的黏附。中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程全文链接：

Silane coupler effects on titanium surface modification and biocompatibility

BACKGROUND:Nowadays, silane coupling for metal surface pretreatment has not been widely applied yet. OBJECTIVE:To observe the surface appearance, structure and biocompatibility of NaOH pre-treated titanium sheet coated with silane films. METHODS:The NaOH pre-treated titanium sheet was silanized with the silane coupler (KH-550) at different concentrations (8%, 15% and 33%). The test specimens manufactured with different concentrations of silane treatment solutions were analyzed with scanning electron microscope and energy dispersive spectroscopy analyzer, in comparison with pure titanium and alkali-pretreated titanium sheets as controls. After silanization, bone marrow mesenchymal stem cells from dogs were co-cultured with the test specimens to observe the cell morphology and adhesion on the surface of test specimens. RESULTS AND CONCLUSION: The silane film consisted of brain shaped sheets which arranged closely. The composition of the silane film was C, N, O and Si. The test specimen coated with the 33% silane solution had the full silane film, while the silane film was imperfect at a concentration of 8%. The cell adhesion was better on the surface of test specimens treated with 33% silane solution than the other specimens. These findings indicate that the titanium sheet modified with 33% silane solution possesses a good biocompatibility, thereby improving the cell adhesion, spreading, proliferation and differentiation.