戊二醛交联对壳聚糖/羟基磷灰石-庆大霉素缓释材料性能的影响

背景：交联是骨组织工程材料改性的一种常用方法，但目前仍缺乏交联剂对载药人工骨材料性能影响的相关研究与报道。目的：研究戊二醛交联对壳聚糖/羟基磷灰石-庆大霉素载药人工骨材料力学性能、降解性能及体外药物缓释行为的影响。方法：分别制备壳聚糖质量分数为10%，20%，30%的壳聚糖/羟基磷灰石-庆大霉素载药人工骨材料与戊二醛交联壳聚糖/羟基磷灰石-庆大霉素载药人工骨材料，检测各组材料的机械强度、吸水率、降解率及体外药物释放行为。结果与结论：壳聚糖含量为10%，20%，30%壳聚糖/羟基磷灰石-庆大霉素的抗压强度分别为(10.16±1.17)，(28.40±0.64)，(23.28±1.30) MPa，经戊二醛交联后材料的抗压强度分别增大至(36.30±1.20)，(51.60±2.08)，(36.90±3.22) MPa。壳聚糖含量为10%，20%，30%壳聚糖/羟基磷灰石-庆大霉素交联后的吸水率与降解率均低于交联前。在体外缓释的第1天，30%壳聚糖/羟基磷灰石-庆大霉素的药物释放量为42.2%，材料经戊二醛交联处理后药物释放量降至33.6%，在随后的9 d，交联壳聚糖/羟基磷灰石-庆大霉素的总释放量均低于壳聚糖/羟基磷灰石-庆大霉素。表明戊二醛交联赋予了材料更好的生物稳定性，减缓了材料降解速率，显著改善了药物突释现象。中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程全文链接：

Effect of glutaraldehyde cross-linking on the properties of chitosan/hydroxyapatite- gentamicin delayed materials

BACKGROUND:Cross-linking is a common method for the modification of bone tissue engineering materials, but there are few studies about the effect of cross-linking on properties of drug-loaded artificial bone.OBJECTIVE:To study the effect of glutaraldehyde cross-linking on mechanical strength, degradation rate and in vitro release behavior of chitosan/hydroxyapatite-gentamicin delayed materials.METHODS:The chitosan/hydroxyapatite-gentamicin drug-loaded artificial bone and the cross-linked chitosan/hydroxyapatite-gentamicin drug-loaded artificial bone were prepared at 10%, 20% and 30% chitosan mass ratio. The mechanical strength, absorption rate, degradation rate and in vitro release behavior of materials in each group were determined.RESULTS AND CONCLUSION: The compressive strength of 10%, 20%, 30% chitosan/hydroxyapatite- gentamicin drug-loaded artificial bone was (10.16±1.17) MPa, (28.4±0.64) MPa and (23.28±1.30) MPa, respectively. After cross-linking, the strength was increased to (36.3±1.20) MPa, (51.6±2.08) MPa and  (36.9±3.22) MPa, respectively. The absorption rate and degeneration rate were reduced by cross-linking. In the first day of drug release in vitro, the rate of gentamicin released from non-cross-linked 30% chitosan/hydroxyapatite-gentamicin drug-loaded artificial bone was 42.2%, while the rate was decreased to 33.6% after cross-linking. At the following 9 days, the total release of the cross-linked material was lower than that of non-cross-linked material. Glutaraldehyde cross-linking could improve the stability, reduce the degradation rate, and significantly ameliorate the release of artificial bone.