Bio-Gide胶原膜对兔骨髓间充质干细胞增殖和成骨分化的影响

背景：相关实验表明Bio-Gide胶原膜与细胞有良好的生物相容性，但有关与其复合培养干细胞成骨分化能力的报道少见。目的：观察Bio-Gide胶原膜对骨髓间充质干细胞增殖及成骨分化的影响。方法：全骨髓贴壁法体外分离培养兔骨髓间充质干细胞，将第3代兔骨髓间充质干细胞分别接种于覆盖Bio-Gide胶原膜的培养板(实验组)与单纯培养板(对照组)培养。于培养1，4，7，14 d利用CCK-8试剂盒检测细胞增殖；成骨分化诱导培养1，4，7，14 d收集细胞培养液上清，检测细胞碱性磷酸酶活性。结果与结论：两组细胞数量均随着培养时间的增加而不断增加，对照组培养7 d细胞数量明显多于实验组(P < 0.05)，其他时间点组间比较差异无显著性意义。两组细胞碱性磷酸酶活性均随着培养时间的增加而不断增加，实验组成骨诱导14 d细胞碱性磷酸酶活性高于对照组(P < 0.05)，其他时间点组间比较差异无显著性意义。表明Bio-Gide胶原膜可促进兔骨髓间充质干细胞的增殖及成骨分化。中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程全文链接：

Effect of Bio-Gide collagen membranes on the proliferation and osteogenetic differentiation of rabbit bone marrow mesenchymal stem cells

BACKGROUND:Bio-Gide collagen membranes show a good biocompatibility with stem cells. But the research on the osteogenetic differentiation of bone marrow mesenchymal stem cells cultured on the Bio-Gide collagen membranes is rarely reported. OBJECTIVE:To observe the effect of Bio-Gide collagen membranes on the proliferation and the osteogenetic differentiation of bone marrow mesenchymal stem cells. METHODS:Bone marrow mesenchymal stem cells from rabbits were isolated and cultured by using the whole bone marrow adherence method in vitro. Passage 3 bone marrow mesenchymal stem cells were selected and seeded on the Bio-Gide collagen membrane pretreated petri dish (experimental group) and simple petri dish (control group). The proliferation of bone marrow mesenchymal stem cells was detected by Cell Counting Kit-8 at 1, 4, 7, 14 days. The supernatant of the cells cultured in osteogenic differentiation medium were collected to detect the activity of alkaline phosphatase at 1, 4, 7, 14 days. RESULTS AND CONCLUSION:The number of bone marrow mesenchymal stem cells in the two groups was increased with the increasing time, and the control group had more cells than the experimental group at 7 days  (P < 0.05). There was no significant difference between the two groups at other time points. The alkaline phosphatase activity was increased with the increasing culture time, and the experimental group had a higher activity than the control group at 14 days (P < 0.05). There was no significant difference between the two groups at other time points. Experimental findings indicate that Bio-Gide collagen membranes can promote the proliferation and the osteogenetic differentiation of bone marrow mesenchymal stem cells.