电针合谷穴对大鼠局灶性脑缺血再灌注后脑内毛细血管密度及CD34表达的影响

目的观测电针大鼠双侧合谷穴(LI04)对局灶性脑缺血再灌注后脑内CD34表达及血管新生的影响。方法90 只大鼠随机分为正常组(n=6)、模型组(n=42)和电针组(n=42)。模型组和电针组采用线栓法制备大鼠局灶性脑缺血再灌注模型，电针组采用电针治疗。局灶性脑缺血1 h 后再灌注，观察再灌注后2 h、12 h、24 h、3 d、7 d、14 d、21 d 共7 个时相点大鼠神经症状学评分，采用HE染色观察并计算梗死体积和毛细血管密度，免疫组织化学法检测CD34的表达。结果电针组与模型组比较，再灌注后各时间点神经症状学评分降低(P<0.05)。电针组梗死体积显著小于模型组(P<0.001)。与模型组相比，电针组毛细血管密度再灌注后2 h 开始增加(P<0.05)，再灌注后24 h 到再灌注后14 d 均较模型组明显增加(P<0.01)。电针组再灌注后3 d CD34阳性细胞表达较模型组明显增加(P<0.01)，7 d 增加达高峰(P<0.01)，14 d 仍有显著性差异(P<0.05)。结论电针能促进局灶性脑缺血再灌注后CD34的表达，增加毛细血管密度，促进血管新生。

Effect of Electroacupuncture at Hegu (LI04) on Capillary Density and Expression of CD34 after Focal Cerebral Ischemia/Reperfusion in Rats

Objective To observe the effect of electroacupuncture at Hegu (LI04) on capillary density and expression of CD34 after focal cerebral ischemia/reperfusion in rats. Methods 90 rats were randomly divided into normal group (n=6), model group (n=42) and electroacupuncture group (n=42). A rat model of focal cerebral ischemia/reperfusion was made by filament occlusion in the model group and the electroacupuncture group. And the electroacupuncture group received electroacupuncture. They were observed 2 h, 12 h, 24 h, 3 d, 7 d, 14 d, 21 d after reperfusion 1 h followed ischemia. HE staining was used to observe and calculate the infarct volume and capillary density, and immunohistology was used to detect the expression of CD34 after neurologic symptoms rating. Results Compared with the model group, the neurologic symptoms score significantly decreased 2 h after reperfusion in the electroacupuncture group (P<0.01), and still decreased 21 d after reperfusion (P<0.05). The infarct volume significantly was smaller in the electroacupuncture group than in the model group (P<0.01). The capillary density was higher 2 h, 24 h, 3 d, 7 d, 14 d after reperfusion in the electroacupuncture group than in the model group (P<0.05). Comparedwith the model group, the expression of CD34 significantly increased 3 d after reperfusion in the electroacupuncture group (P<0.01),and it reached a peak at 7 d after reperfusion (P<0.01), and still higher at 14 d. Conclusion Electroacupuncture can promote the expression of CD34 and the capillary density, and stimulate the angiogenesis after focal ischemia/reperfusion.