大鼠成骨细胞与壳聚糖/羟基磷灰石复合支架降解产物的生物相容性

背景：人们对壳聚糖/羟基磷灰石复合多孔生物支架在体内的降解过程并非十分清楚，而且有关其降解产物对成骨细胞的影响研究也较少。 目的：分析大鼠成骨细胞与壳聚糖/羟基磷灰石复合多孔生物支架降解产物的生物相容性。 方法：将培养的第2代大鼠成骨细胞分别在壳聚糖/羟基磷灰石复合支架降解产物浸提液和含体积分数10%胎牛血清的DMEM培养液中培养，培养第2，4，6，8，10天分别对两组细胞做MTT细胞计数，采用联合会推荐法测定细胞碱性磷酸酶活性，采用BCA蛋白定量法测定总蛋白。 结果与结论：在壳聚糖/羟基磷灰石复合多孔生物支架降解产物浸提液中培养的大鼠成骨细胞增殖速度、细胞碱性磷酸酶活性、细胞总蛋白合成及碱性磷酸酶与总蛋白的比值明显高于在体积分数为10%胎牛血清DMEM培养液中培养的细胞(P < 0.05)。表明壳聚糖/羟基磷灰石复合多孔生物支架的降解产物不仅可促进大鼠成骨细胞的黏附、生长和增殖，还可增强其骨化功能，具有较好的生物相容性。中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程全文链接：

Biocompatibility of rat osteoblasts with chitosan/hydroxyapatite composite scaffold degradation products

BACKGROUND:The in vivo degradation process of chitosan/hydroxyapatite composite porous scaffolds is not very clear. Research on the effects of rat osteoblasts and degradation products is less. OBJECTIVE:To analyze the biocompatiblity of rat osteoblasts with degradation products of chitosan/hydroxyapatite composite porous scaffolds. METHODS:The second generation of cultured rat osteoblasts were respectively cultured in the extract of degradation products of chitosan/hydroxyapatite composite scaffolds (experimental group) and Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum (control group). At 2, 4, 6, 8, 10 days of culture, cell counting was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Alkaline phosphatase activity was measured by the recommended method of determination of the Federation, and total protein was determined by BCA method. RESULTS AND CONCLUSION:The proliferation speed, alkaline phosphatase activity, total cellular protein synthesis and ratio of alkaline phosphatase to total protein in rat osteoblasts cultured in the experimental group were significantly higher than those in the control group (P < 0.05). This experiment showed that the degradation products of chitosan/hydroxyapatite composite porous scaffolds cannot only promote rat osteoblast adhesion, growth and proliferation, but also enhance its ossification function, with good biocompatibility.