纳米含氟羟基磷灰石牙种植体的生物相容性

背景：有关纳米含氟羟基磷灰石牙种植体材料生物相容性的报道较少。 目的：检测纳米含氟羟基磷灰石牙种植体材料的体外生物相容性。 方法：采用溶胶凝胶技术分别制备羟基磷灰石与纳米含氟羟基磷灰石。①溶血性实验：在0.2 mL稀释兔抗凝血中分别加入0.01，0.15，0.2 g/L纳米含氟羟基磷灰石溶液、生理盐水及蒸馏水各10 mL，检测各组上清液吸光度值。②体外细胞毒性实验：分别以100%，50%纳米含氟羟基磷灰石浸提液、100%羟基磷灰石浸提液、苯酚溶液及RPMI1640培养液培养传至第2代的L929细胞，MTT法检测培养2，4，7 d的吸光度值。 结果与结论：体外溶血性实验显示，各浓度梯度纳米含氟羟基磷灰石的溶血率均在5%以内，符合医用材料的溶血要求。体外细胞毒性实验显示，随着培养时间的增加，100%，50%纳米含氟羟基磷灰石浸提液组细胞贴壁覆盖率增加，细胞密度增高，细胞为长梭形或多角形，细胞增殖及形态与RPMI1640培养液组、羟基磷灰石组无明显差别，细胞毒性为0级。中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程全文链接：

Biocompatibility of nano fluoridated hydroxyapatite for dental implants

BACKGROUND:Few reports focus on biocompatibility of nano fluoridated hydroxyapatite for dental implants. OBJECTIVE:To investigate the biocompatibility in vitro of nano fluoridated hydroxyapatite modified for dental implants. METHODS:We utilized sol-gel method to prepare nano fluoridated hydroxyapatite and nanohydroxyapatite powders. (1) Hemolysis test: 0.01, 0.15, 0.2 g/L nano fluorinated hydroxyapatite solution, saline and distilled water at a volume of 10 mL were added into 0.2 mL diluted rabbit anti-coagulation blood samples, respectively. Then the supernatant was detected by absorbance values. (2) In vitro cytotoxicity test: Passage 2 L929 cells were respectively cultured in culture media containing 100%, 50% nano fluorinated hydroxyapatite extract, 100% hydroxyapatite extract, phenol solution and RPMI1640. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was employed to measure absorbance values at days 2, 4, 7 of culture. RESULTS AND CONCLUSION:Hemolysis test in vitro showed the hemolysis rates of nano fluorinated hydroxyapatite groups were less than 5%, which were accorded with haemolysis demand of medical materials. The cytotoxicity test in vitro showed during cultivation, the adherence rate of L929 cells cultured in 100% and 50% nano fluorinated hydroxyapatite extracts were increasing and cell density was rising up. Cells were fusiform or polygon, which had no evident differences from negative controls in morphology. Nano fluorinated hydroxyapatite showed nontoxic to L929 cells in vitro.