经皮纤维环穿刺与经肌间隙纤维环刀刺建立兔椎间盘退变模型

背景：有研究表明椎间盘退变模型的建立可为椎间盘退变治疗提供实验载体，但目前尚缺乏公认的最佳实验动物模型。目的：比较经皮纤维环穿刺法和经肌间隙纤维环刀刺法建立兔椎间盘退变模型的差异。方法：将新西兰大白兔分别采用经皮纤维环穿刺法和经肌间隙纤维环刀刺法建立兔椎间盘退变模型，穿刺后4，8，16周通过磁共振和组织病理学检查观察腰椎间盘髓核变性及组织病理情况。结果与结论：穿刺后4周，两组兔椎间盘髓核内T2加权像信号降低、变暗，椎间隙高度下降，但经皮纤维环穿刺组T2信号强度评分较经肌间隙纤维环刀刺组低(P < 0.05)；穿刺后8周，两组T2信号强度评分均增高，经皮纤维环穿刺组T2信号强度评分较经肌间隙纤维环刀刺组低(P < 0.05)；穿刺后16周，两组兔椎间盘髓核内T2信号强度评分达最高且差异无显著性意义，两组椎间隙均明显变窄，椎间盘均亮度变黑，两组差异不明显；经皮纤维环穿刺组手术时间少于经肌间隙纤维环刀刺组(P < 0.05)，经肌间隙纤维环刀刺组感染率为5.6%，而经皮纤维环穿刺组无感染。结果证实，经皮纤维环穿刺法建立兔椎间盘退变模型建模时间短，感染率低，效果优于经肌间隙纤维环刀刺法造模。中国组织工程研究杂志出版内容重点：人工关节；骨植入物；脊柱；骨折；内固定；数字化骨科；组织工程全文链接：

Percutaneous annulus fibrosus needle puncture versus muscular annulus fibrosus knife penetration for establishing a rabbit model of intervertebral disc degeneration

BACKGROUND: Studies have shown that the establishment of disc degeneration model provides experimental support for the treatment of intervertebral disc degeneration, but the current study still lacks of recognized optimal animal models. OBJECTIVE: To compare the differences of percutaneous annulus fibrosus needle puncture with muscular annulus fibrosus knife penetration in establishing a rabbit model of intervertebral disc degeneration.METHODS: The New Zealand rabbit models of intervertebral disc degeneration were established by percutaneous annulus fibrosus needle puncture and muscular annulus fibrosus knife penetration. At 4, 8 and 16 weeks after puncture, lumbar disc degeneration and histopathological conditions were observed using magnetic resonance imaging and histopathology examination. RESULTS AND CONCLUSION: At 4 weeks after the puncture, T2-weighted image signal reduced, darken, and disc height decreased in rabbit nucleus pulposus of the two groups. T2 signal intensity scores were lower in the percutaneous annulus fibrosus needle puncture group compared with the muscular annulus fibrosus knife penetration group (P < 0.05). At 8 weeks after puncture, T2 signal intensity scores were increased in both groups. T2 signal intensity scores were lower in the percutaneous annulus fibrosus needle puncture group compared with the muscular annulus fibrosus knife penetration group (P < 0.05). At 16 weeks after puncture, T2 signal intensity reached a peak, and no significant difference was detected between the two groups. Intervertebral space became significantly narrowed. The brightness of the disc became black. There were no significant differences between  the two groups. The time of operation was shorter in the percutaneous annulus fibrosus needle puncture group than that in the muscular annulus fibrosus knife penetration group (P < 0.05). Infection rate was 5.6% in the muscular annulus fibrosus knife penetration group. No infection was observed in the percutaneous annulus fibrosus needle puncture group. Results verified that the time of establishing a rabbit model of intervertebral disc degeneration using percutaneous annulus fibrosus needle puncture was short, and the infection rate was low. Thus, its effects were better than muscular annulus fibrosus knife penetration.