不同浓度人羊膜匀浆上清液培养大鼠许旺细胞的增殖

背景：许旺细胞是周围神经修复过程的重要细胞，而研究发现人羊膜细胞分泌的多种细胞因子能够促进许旺细胞增殖。 目的：观察不同浓度人羊膜匀浆上清液对鼠许旺细胞(RSC96)生长的影响。 方法：使用含体积分数20%胎牛血清的高糖DMEM培养基原代培养RSC96细胞株，传代至第2代用于实验研究。根据人羊膜匀浆上清液在培养基中的不同体积分数(0，10，15，20，25%)分组。 结果与结论：人羊膜匀浆上清液的总蛋白浓度为675 mg/L，表皮生长因子、碱性成纤维生长因子和血管内皮生长因子浓度分别为(470.625±2.546)，(4.121±0.026)和(0.172±0.002) ng/L。在培养第1-7天，10%和15%人羊膜匀浆上清液组的增殖率大于20%和25%人羊膜匀浆上清液组(P < 0.05)；10%、15%人羊膜匀浆上清液组显示出促进细胞增殖的作用，而20%、25%人羊膜匀浆上清液组显示出抑制细胞增殖的作用；各实验组的细胞活力与对照组接近(P > 0.05)。提示人羊膜匀浆上清液低浓度时(10%和15%)具有促进RSC96增殖作用，高浓度时(20%和25%)抑制RSC96增殖。 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程全文链接：

Effect of different concentrations of human amniotic homogenate supernatant on the proliferation of rat Schwann cells

BACKGROUND: Schwann cells are important cell lines in the process of repairing peripheral nerve injury, and human amniotic homogenate supernatant is shown to secrete a variety of cytokines, which could promote the proliferation of Schwann cells. OBJECTIVE: To investigate the effect of different concentrations of human amniotic homogenate supernatant on the proliferation of rat Schwann cell 96. METHODS: Schwann cell 96 was cultured with high-glucose DMEM containing 20% fetal bovine serum, and the second generation of Schwann cell 96 was applied for experiments. The cultured cells were divided into five groups according to different volume fractions of human amniotic homogenate supernatant (0%, 10%, 15%, 20%, 25%) in the medium.  RESULTS AND CONCLUSION: The total protein concentration of human amniotic homogenate supernatant was 675 μg/mL, in which the concentration of epidermal growth factor, basic fibroblast growth factor and vascular endothelial growth factor were respectively (470.625±2.546), (4.121±0.026) and (0.172±0.002) ng/L. At 1-7 days, the cell proliferation rate of the 10% and 15% concentration groups was greater than that in 20% and 25% concentration groups (P < 0.05); 10% and 15% concentrations promoted cell proliferation, while 20% and 25% concentrations inhibited cell proliferation. There were no significant difference in the viability of Schwann cell 96 between the control group and the experimental group (P > 0.05). Low concentrations (10%, 15%) of human amniotic homogenate supernatant promote the proliferation of Schwann cell 96, while high concentrations (20%, 25%) of human amniotic homogenate supernatant inhibit cell proliferation.