| 胰岛素抵抗大鼠葡萄糖-6-磷酸酶的活性及基因表达变化 |
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| 引用本文： | 李全民,张素华,任伟,吴静,倪银星. 胰岛素抵抗大鼠葡萄糖-6-磷酸酶的活性及基因表达变化[J]. 实用医学杂志, 2005, 21(22): 2487-2490 |
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| 作者姓名： | 李全民 张素华 任伟 吴静 倪银星 |
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| 作者单位： | 1. 100088,北京市,解放军第二炮兵总医院内分泌科
2. 400016,重庆医科大学附属第一医院内分泌科 |
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| 摘 要： | 目的:探讨高脂饮食致胰岛素抵抗动物模型中,葡萄糖-6-磷酸酶(G-6-Pase)的活性和基因表达改变与胰岛素抵抗的关系。方法:Wistar大鼠16只,随机分为2组,分别给予普通饲料和高脂饲料喂养,制作胰岛素抵抗模型。6周后测定各组体重、血糖、血脂、胰岛素变化,并利用高胰岛素-正常葡萄糖钳夹术评价胰岛素敏感性。测定肝脏微粒体G-6-Pase的活性和基因表达。结果:高脂饲料组大鼠体重、甘油三酯、游离脂肪酸和胰岛素水平显著增高,G-6-Pase活性增高[(0.51±0.11)vs(0.32±0.11)μmol/(min·mgprot),P<0.05],两组间的G-6-Pase基因表达无差异。结论:G-6-Pase参与了肝胰岛素抵抗的发病机制。
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| 关 键 词： | 胰岛素抗药性 葡糖磷酸脱氢酶 大鼠 基因 |
| 收稿时间： | 2005-07-05 |
| 修稿时间： | 2005-07-05 |
Changes of glucose-6-phosphatase activities and the gene expressions in fat-fed rats of insulin resistance |
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| LI Quan-min,ZHANG Su-hua,REN Wei,WU Jing,NI Yin-xing. Changes of glucose-6-phosphatase activities and the gene expressions in fat-fed rats of insulin resistance[J]. The Journal of Practical Medicine, 2005, 21(22): 2487-2490 |
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| Authors: | LI Quan-min ZHANG Su-hua REN Wei WU Jing NI Yin-xing |
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| Affiliation: | LI Quan-min*,ZHANG Su-hua,REN Wei,WU Jing,NI Yin-xing.* Department of Endocrinology,the Second Artillery General Hospital of PLA,Beijing 100088,China |
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| Abstract: | Objective To explore the activities and gene expressions of glucose-6-phosphatase in fat-fed rats of insulin resistance. Methods sixteen rats were randomly divided into two groups(each group 8 rats). One group was given standard food and the other group was given high-fat diet to make insulin resistance model for 6 weeks. Insulin sensitivity was valued by hyperinsulinemic euglycemic clamp. The activities of glucose-6-phosphatase were measured and the mRNA expressions of the enzyme were assessed by RT-PCR. R... |
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| Keywords: | Insulin resistance Glucosephosphate dehydrogenase Rats Genes |
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