神经生长因子脑室内注射对脑积水幼鼠的行为改变的影响

目的 研究神经生长因子脑室内注射对脑积水幼鼠的行为改变的影响。方法 雄性Wistar 1月龄大鼠30只，全部采用枕大池注射白陶土的方法制备交通性脑积水的模型，1个月后行MRI证实。模型制备后将脑积水幼鼠随机分成3组，每组10只，实验组脑室内注入神经生长因子(NGF)0.3 ml(1000 U，连续3 d)，安慰组脑室内注入生理盐水0.3ml（连续3 d），对照组不做任何处理。2周后对3组幼鼠分别进行Morris水迷宫和旷场分析进行研究，实验后处死脑积水幼鼠，常规HE染色及免疫组织化学检测海马区结构变化及GFAP与S-100β的表达。结果 安慰组和对照组脑积水幼鼠游泳所用时间(108.1±31.2，110.2±28.7)及错误次数(4.8±1.4，4.7±1.5)均多于实验组(82.1±21.3，3.3±1.3)，空间认知能力及新环境适应性低于实验组。各组脑积水幼鼠海马区均有GFAP的阳性表达，实验组122.53±13.50与安慰组224.68±8.65及对照组256.80±10.22间差异有统计学意义。各组脑积水幼鼠海马区均可见S-100β阳性细胞存在，安慰组及对照组海马区可见大量阳性细胞染色，与实验组比较差异有显著统计学意义。结论 安慰组和对照组脑积水幼鼠学习记忆能力、空间认知能力及兴奋性均低于实验组，表明神经生长因子脑室内注射对脑积水后脑损伤有一定的脑保护作用。

The effect of nerve growth factor injection in cerebral ventricle on the behavior change of hydrocephalus young rats

Objective To study the effect of nerve growth factor injection in cerebral ventricle on the behavior change of hydrocephalus young rats. Methods Thirty 1-month-old male Wistar rats,all treated by injection of white clay into cistema magna to develop the models of communicating hydrocephalus, were verified 1 month later by MRI. After the models gained, the rats were randomly divided into three groups, the experimental group was injected NGF 0. 3 ml (1000U, for 3 days consecutively) in cerebral ventricle, the placebo group was injected 0. 3 ml physiological saline (for 3 days consecutively) in cerebral ventricle, the control group was received no treatment. Two weeks later,the three groups of rats' behavior were studied respectively by Morris water maze and open-field analysis, then the rats were sacrificed after experiment. And the structural changes in hippocampus of rats and the expressions of S-100β and GFAP were shown by HE staining and immunohistochemistry. Results The rats in placebo group and control group had longer swimming time (108. 1 ± 31.2 vs 110. 2± 28. 7), and more errors in water maze (4. 8 ± 1.4 vs 4. 7 ± 1.5), but lower the capability in spatial cognition and adaptability to new environment (82. 1 ± 21.3 vs 3.3 ± 1.3). The positive expression fo GFAP in hippocampus of rats in experimental group(122. 53 ± 13. 50) was significant lower than that in the placebo group(224. 68 ± 8. 65) and control group(256. 80 ± 10. 22). The S-1003-positive cells in hippocarnpus of rats in experiment group were also siginifcant lower than those of the placebo group and control group. Conclusions The learning and memorizing ability, spatial cognition ability and activity in placebo group and control group of young rats are lower than those in experimental group,showing that the injection of nerve growth factor in cerebral ventricle has protective function to the hydrocephalus cerebellum damage.