| CHO细胞中VMAT2的鉴定及应用 |
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| 引用本文： | 王铭维,刘力,顾平,崔冬生,王彦永.CHO细胞中VMAT2的鉴定及应用[J].河北医科大学学报,2008,29(6). |
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| 作者姓名： | 王铭维 刘力 顾平 崔冬生 王彦永 |
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| 作者单位： | 1. 河北医科大学第一医院神经内科,河北,石家庄,050031
2. 河北省脑老化与认知神经学实验室,河北,石家庄,050031 |
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| 摘 要： | 目的鉴定转染囊泡单胺转运体(vesicular monoamine transporter-2,VMAT2)基因在中国仓鼠卵巢细胞(Chinese hamster ovary cell,CHO)细胞中VMAT2的表达情况。方法采用细胞免疫荧光染色、RT-PCR、免疫电镜的方法,分别从形态学、分子生物学和微观亚细胞结构角度来确定VMAT2的表达。结果免疫荧光染色显示VMAT2-CHO表达明亮的绿色荧光;逆转录聚合酶链反应电泳显示VMAT2-CHO出现180 bp的阳性条带;VMAT2-CHO细胞中免疫胶体金颗粒分布在细胞核周膜性结构上。结论转染VMAT2基因的CHO细胞可以稳定且大量表达VMAT2,有望成为研究VMAT2功能与帕金森病关系的单一的细胞模型。
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| 关 键 词： | 帕金森病 转染 仓鼠 |
IDENTIFICATION AND APPLICATION OF VESICULAR MONOAMINE TRANSPORTER-2 IN CHO CELL |
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| WANG Ming-wei,LIU Li,GU Ping,CUI Dong-sheng,WANG Yan-yong.IDENTIFICATION AND APPLICATION OF VESICULAR MONOAMINE TRANSPORTER-2 IN CHO CELL[J].Journal of Hebei Medical University,2008,29(6). |
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| Authors: | WANG Ming-wei LIU Li GU Ping CUI Dong-sheng WANG Yan-yong |
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| Abstract: | Objective To identify the expression of vesicular monoamine transporter-2(VMAT2) in Chinese hamster ovary cell(CHO) cell.Methods Immunofluorescence,RT-PCR and immunoelectron microscope were used to analyze the VMAT2 expression and location in CHO cells.Results There was bright green fluorescence in VMAT2-CHO,Agarose electrophoresis of RT-PCR products displayed the 180 bp positive band in the VMAT2-CHO,There were a little colloidal gold particles around the nucleus.Conclusion VMAT2-CHO can stably express the VMAT2 protein and can be hope to used to study the function of VMAT2 and Parkinson's disease. |
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| Keywords: | parkinson disease transfection hamsters |
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