卵胞浆单精子注射中异常原核受精卵的体外发育及遗传多态性

目的 比较卵胞浆内单精子注射(intracytoplasmic sperm injection,ICSI)治疗周期中异常原核受精卵的发育,并分析其遗传多态性.方法 分别收集ICSI中未见原核(nonpronuclear,0PN)成熟卵347个、单原核(monopronuclear,1PN)受精卵71个、多原核(multipronuclear,≥3PN)受精卵75个,在Vitro1ife公司G5系列胚胎培养液中分组体外培养.复合扩增7个发育为囊胚的异常原核胚胎细胞16个短串联重复序列(short tandem repeat,STR)基因座,在ABI3100遗传分析仪上检测其遗传多态性.结果 0PN组卵裂率为25.4%,显著低于其他两组,且组间差异有统计学意义(P＜0.01);胚胎停育率0PN组为48.9%,显著高于其他两组(P＜0.05);各组囊胚形成率差异无统计学意义(P＞0.05).ABI3100遗传分析仪电泳图谱显示,0PN组和≥3PN组被检测囊胚细胞STR基因座均显示为同源染色体组成二倍体特性,且0PN组囊胚细胞囊存在Y染色体特异性STR基因座.结论 ICSI治疗周期中异常原核受精卵有继续发育的潜能,发育到囊胚的0PN受精卵和≥3PN受精卵可能为正常二倍体胚胎.

Development and genetic polymorphism of abnormal pronuclear zygotes after intracytoplasmic sperm injection

Objective To compare the development of abnormal pronuclear zygotes after intracytoplasmic sperm injection (ICSI) and analyze their genetic polymorphism. Methods Four hundred and ninety three abnormal pronuclear zygotes after ICSI were divided into three groups based on the number of pronuclei: 347 nonpronuclear oocytes, 71 monopronuclear zygotes and 75 multipronuclear zygotes. All of them were cultured in the medium of Vitrolife G5 seriesTM. Sixteen short tandem repeats(STR) of seven blastocysts were then analyzed by ABI3100. Results The cleavage rate of nonpronuclear group (25.4%)was lower than that of the others (P＜0. 01), the proportion of blocked embryos in nonpronuclear group (48.9%) was significantly higher than that of the others (P＜0.05), but the blastocyst rate showed no significant difference in three groups (P＞0. 05). The genetic polymorphism of the 16 STRs showed that the blastocysts from the nonpronuclear and multipronuclear were diploid, and one of the blastocysts from nonpronuclear oocyte was Y-bearing. Conclusion The zygotes with abnormal pronuclei after ICSI might have development potential, and the blastocysts from nonpronuclear oocytes and multipronuclear zygotes could be diploid.