碱性“彗星”法检测肺癌放射敏感性初步研究

目的采用碱性彗星法检测肺癌标本的初始DNA单链断裂,探讨碱性彗星法用于预测临床肺癌的放射敏感性的可行性。方法对2002年4月~2003年8月在本院经纤维支气管镜取样并为病理证实的150例肺癌标本采用碱性彗星法检测初始DNA单链断裂,以经本底较正的平均尾力矩(RTM)和平均中位尾力矩(mRTM)为评价指标;采用SPSS 10.0统计软件包以单因素方差分析法比较不同病理类型平均较正尾力矩差异,并分析影响结果的相关因素。结果纤维支气管镜取样,多数样品细胞数较少;样品质量对结果影响较大,35%样品中位RTM≤1.0,无显著性差异,全组平均较正尾力矩在不同病理类型中的分布均未显示出与临床一致的趋势,在细胞数小于100的样品中可见小细胞大于非小细胞的趋势;全组平均中位较正尾力矩尤其在中位RTM>1.0的样本中,显示与临床放射反应一致分布趋势,但样本小,结果无统计学差异。结论碱性彗星法可初步显示不同病理类型肺癌细胞间的放射敏感性差异,样品质量对结果影响较大,分析方法有待改进完善。

PRELIMINARY RESULTS OF RADIOSENSITIVITY DETECTED BY ALKALINE COMET ASSAY IN LUNG CANCER PATIENTS

Objective To evaluate the value of alkaline comet assay for predicting clinical radio-sensitivity of lung cancer patients.Methods The biopsy samples from 150 lung cancer patients by fibrous bronchial endoscopy were tested by alkaline comet assay from April,2002 to August,2003. The adjusted median tail moment(mRTM) and adjusted mean median tail moment(RTM) were used as the endpoints.SPSS 10.0 software was used to analyse the difference in the mRTM and RTM among different pathologic types by ANOVA.Factors influencing the result were also analysed.Results There were more samples with less cells scored due to biopsy by fibrous bronchial endoscopy.The quality of the samples has a greaet influence on the results.Thirty five percent of the samples with mRTM≤1.0 are recognized as unreasonable results.The results derived from the samples with number of cells less than 100 show a tend of RTM in small cell lung carcinoma greater than that in non?small cell lung carcinoma.There was the same distribution trend of the RTM among three pathologic types as that of the clinic irradiation response for all samples especially for the samples with RTM>1.0,but do not reach the statistic discrepancy due to a less sample size.Conclusions The preliminary results from alkaline comet assay may reveal the difference in radiosensitivity among three different pathologic types.The quality of samples influences greatly on reliability of the results and the assay should be improved further.