| B细胞趋化因子对CVB3融合基因疫苗pcDNA3/C3d3-sVP1免疫效果的影响 |
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| 引用本文： | 高志云,揣侠,蓝佳明,刘贵霞,李剑,张永红,王永祥. B细胞趋化因子对CVB3融合基因疫苗pcDNA3/C3d3-sVP1免疫效果的影响[J]. 中国免疫学杂志, 2010, 26(2) |
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| 作者姓名： | 高志云 揣侠 蓝佳明 刘贵霞 李剑 张永红 王永祥 |
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| 作者单位： | 河北医科大学病原生物学教研室,石家庄,050017 |
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| 基金项目： | 河北省科学技术研究与发展计划项目 |
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| 摘 要： | 目的:探讨B细胞趋化因子(B-lymphocyte chemoattractant,BLC)对柯萨奇病毒B3融合基因疫苗pcDNA3/C3d3-sVP1免疫效果的影响。方法:将BALB/c小鼠随机分为4组,分别肌肉注射pcDNA3、pcDNA3/BLC、pcDNA3/C3d3-sVP1和含pcDNA3/BLC与pcDNA3/C3d3-sVP1的混合质粒,于免疫后不同时间检测小鼠血清的中和抗体滴度、特异性CTL杀伤活性;以LD50的CVB3病毒液感染已免疫小鼠,检测小鼠血中病毒滴度,观察存活情况以评价各种疫苗的免疫保护作用。结果:小鼠的中和抗体滴度随免疫次数增加而提高,混合组中和抗体滴度(42.17±1.43)和特异性CTL杀伤率(41.3%±3.51%)均明显高于pcDNA3/C3d3-sVP1组(P0.05),而血中病毒滴度低于pcDNA3/C3d3-sVP1组;经致死量CVB3感染后,pcDNA3/BLC和pcDNA3/C3d3-sVP1混合免疫的小鼠生存率达44%,生存率高于其他各组。结论:BLC可显著提高C3d3-sVP1基因诱导的特异性免疫应答,增强基因疫苗对机体的免疫保护作用。
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| 关 键 词： | B细胞趋化因子 补体3d 柯萨奇病毒B3 基因疫苗 |
The influence of B-lymphocyte chemoattractant on the immune response of CVB3 fusion gene vaccine pcDNA3/C3d3-sVP1 |
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| GAO Zhi-Yun,CHUAI Xia,LAN Jia-Ming,LIU Gui-Xia,LI Jian,ZHANG Yong-Hong,WANG Yong-Xiang. The influence of B-lymphocyte chemoattractant on the immune response of CVB3 fusion gene vaccine pcDNA3/C3d3-sVP1[J]. Chinese Journal of Immunology, 2010, 26(2) |
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| Authors: | GAO Zhi-Yun CHUAI Xia LAN Jia-Ming LIU Gui-Xia LI Jian ZHANG Yong-Hong WANG Yong-Xiang |
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| Affiliation: | GAO Zhi-Yun,CHUAI Xia,LAN Jia-Ming,LIU Gui-Xia,LI Jian,ZHANG Yong-Hong,WANG Yong-Xiang.Department of Pathogenic Biology,Hebei Medical University,Shijiazhuang 050017,China |
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| Abstract: | Objective:To investigate the influence of B-lymphocyte chemoattractant on the immune response of CVB3 fusion gene vaccine pcDNA3/C3d3-sVP1.Methods:BALB/c mice were divided into 4 groups randomly,and injected intramuscularly with pcDNA3,pcDNA3/BLC,pcDNA3/C3d3-sVP1 and the combination with the plasmid pcDNA3/BLC and pcDNA3/C3d3-sVP1.At a certain time,they were measured for the titers for neutralizing antibodies,specific CTL cytotoxic activity.The protective efficacy of DNA vaccinations was evaluated by titers... |
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| Keywords: | B-lymphocyte chemoattractant Complement 3d Coxsackieviruses B3 Gene vaccine |
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