CyclinD1、P16和P21WAF1/CIP1在胃癌中的表达意义

目的 :探讨癌基因CyclinD1,抑癌基因p16、p2 1与胃癌发生的生物学行为的关系。方法 :采用免疫组化LSAB法测定 43例胃癌CyclinD1、P16和P2 1WAF1/CIP1蛋白的表达。结果 :CyclinD1在胃癌组织中的阳性率为 5 5 .81% ,CyclinD1与胃癌分化程度呈正相关 (P <0 .0 5 ) ;P16和P2 1在胃癌组织中阳性率分别为 48.84%和 46.5 1% ,P16和P2 1与胃癌分化程度均呈负相关 (P <0 .0 5 ) ;CyclinD1与P16、P2 1分别呈负相关 ;P16与P2 1无相关性 ;CyclinD1、P16和P2 1与胃癌的预后相关。结论 :CyclinD1的高表达 ,P16和P2 1的低表达在胃癌的发生发展中起重要作用 ,并可能成为研究胃癌发生、发展的有用指标。

Expression of CyclinD1,P16 and P21 protein in human gastric carcinoma

Objective To investigate the expression of CyclinD 1,P16 and P21 protein in human gastric carcinoma,to explore the correlation of these proteins expression with biologic behavior of tumors.Method CyclinD 1,P16 and P21 protein expression were studied in formalin fixed,paraffin embedded sections of gastric carcinoma by means of a specific and sensitive immunohistochemical LSAB assay.Results The positive rate of CyclinD 1 in gastric carcinoma tissuse was 55.81%.The expression of CyclinD 1 was positively correlated with grade of tumor differentiation( P <0.05).The positive rate of P16 in gastric carcinoma tissuse was 48.84%.The positive rate of P21 in gastric carcinoma tissuse was 46.51%.The presence of P16 and P21 was negatively correlated with grade of tumor differentiation( P <0.05).There are a high negatively correlation between the expression of CyclinD 1 and P16,between CyclinD 1 and P21.P16 and P21 were non relation.The expression of CyclinD 1,P16 and P21 protein were correlated with the prognosis of patients with gastric carcinoma.Conclusion The overexpression of CyclinD 1,and depressed expression of P16 and P21 are contributing to the occurrence and development of gastric carcinoma.and they can be useful parameteres in studying the occurrence and development of gastric carcinoma.