全反式维甲酸对糖尿病大鼠足细胞损伤的保护作用

目的探讨全反式维甲酸(ATRA)对糖尿病大鼠足细胞损伤的保护作用。方法24只链脲佐菌素(STZ)诱导的糖尿病大鼠模型,随机分为糖尿病组(DM)和治疗组(T)各12只,另以12只正常大鼠作为对照组(N)。T组给予ATRA 20 mg·kg-1·d-1灌胃。于第4、8周末检测各组大鼠尿蛋白定量(24 h)、Cer、肾重/体重、尿中足细胞以及肾脏病理改变。结果DM组尿蛋白定量(24 h)、Ccr、肾重/体重、尿中足细胞数[8周时,0．84(0．60～1．50)个/ml]均显著高于同期N组[0．03(0-0．15)个/m1],而肾小球足细胞数(8周时,11．27±2．15)显著低于同期N组(14．07±2．07),且足细胞足突增宽、融合。T组尿蛋白定量(24 h)、Cer、肾重/体重、尿中足细胞数较同期DM组显著降低[0．46(0．25～0．70)个/ml],且病理改变减轻。结论ATRA可以减少糖尿病大鼠尿足细胞的排泄、降低尿蛋白,对肾脏足细胞损伤具有保护作用。

Protective effect of all-trans retinoic acid on podocyte lesion in diabetic rats

Objective To investigate the protective effect of all-trans retinoic acid (ATRA) on podocyte lesion in diabetic rats. Methods Twenty-four rats injected with streptozotocin(STZ) were randomly divided into model(DM) and therapy group(T). Other 12 normal rats were used as control group(N). The rats in T group were administrated ATRA at a dosage of 20 mg·kg-1·d-1， and animals in other groups were treated with normal saline. 24 h urinary protein， urinary podocyte excretion， and renal pathological alteration were determined after 4 and 8 weeks. Results 24 h urinary protein， ratio of kidney weight to body weight， and urinary podocyte excretion were significantly higher in DM group compared to N group[0.84(0.60~1.50) vs 0.03(0~0.15)cell/ml，P < 0.05]. The numbers of glomerular poddocyte were significantly reduced in DM group(11.27±2.15 vs 14.07±2.07). Diabetic rats treated with ATRA (T group) had less urinary podocyte excretion[0.46(0.25~0.70)cell/ml] and more glomerular podocyte numbers compared to DM group. Conclusions ATRA can decrease the excretion of urinary podocyte and proteinuria. ATRA has a protective effect on podocyte lesion in diabetic rats.