男性铅作业工人生殖内分泌变化的研究

目的探讨铅对男性工人性激素水平的改变及其影响因素。方法选153名职业性接触铅男工为暴露组,同时选择年龄和经济条件相近,距离冶炼车间1.5km煤场中47名不接触铅男工为非铅暴露组,用石墨炉原子吸收光谱法测定血铅,ELISA方法测定血清中睾酮(T)、黄体生成素(FSH)、卵泡刺激素(LH)、雌二醇(E2)和抑制素B水平。结果暴露组男工血铅水平为370.2μg/L,高于非暴露组221.2μg/L(P0.01);暴露组男工血清中T的平均水平为8.20μg/L,低于非暴露组9.65μg/L(P0.05);抑制素B平均水平为240.5pg/ml高于非暴露组211.1pg/ml(P0.05);黄体生成素(FSH)、卵泡刺激素(LH)、雌二醇(E2)水平两组间差异无显著性(P0.05)。通过Logistic回归分析,发现影响血清T水平异常变化的因素主要是年龄和铅暴露,而抑制素B水平的改变主要与铅暴露有关。结论职业性铅暴露能诱导男工血清中T和抑制素B水平的改变,提示接触铅可损伤内分泌和睾丸支持细胞功能。

Effect of lead exposure on male sexual hormone

Objective To explore effects of lead exposure on the concentrations of serum male sex hormone in male workers.Methods Blood sample of 153 male workers occupationally exposed to lead and 47 male worker without exposure history were collected,of which the concentrations of testosterone(T),luteinizing hormone(LH), follicle stimulating hormone(FSH),estradiol( E2 ) and inhibin B were measured by ELISA.Results The results showed that concentration of T in exposure group were significantly lower (P < 0.05) compared with non-exposured group.In contrast,serum inhibin B concentration were significantly increased (P < 0.05) than control group.The main influencing factors changing serum T are effect of lead and age,meanwhile positive effects of lead were also observed on serum inhibin B increased by Logistic analysis.Conclusion The results suggest that the lead exposure may alter the male sexual hormone,which might injure the endocrine function and Sertoli cells.