维生素E和硒对大鼠肝细胞和储脂细胞的影响

目的：　研究维生素Ｅ（ＶＥ）、硒（Ｓｅ）及二者联合应用对四氯化碳（ＣＣｌ４）作用下肝细胞、储脂（Ｉｔｏ）细胞、细胞外基质（ＥＣＭ）产生的抑制作用。方法：　采用大鼠肝脏原位灌流、分离培养肝细胞和Ｉｔｏ 细胞。结果：　ＶＥ、Ｓｅ 能明显抑制ＣＣｌ４ 作用下大鼠肝细胞、Ｉｔｏ 细胞３Ｈ－脯氨酸（３Ｈ－Ｐｒｏ）掺入、Ⅲ型前胶原（ＰＣⅢ）分泌，还可使Ｉｔｏ 细胞透明质酸（ＨＡ）、板层素（ＬＮ）、纤维连接素（ＦＮ）分泌减少。ＶＥ加Ｓｅ在抑制肝细胞、Ｉｔｏ 细胞３Ｈ－Ｐｒｏ 掺入、降低丙二醛（ＭＤＡ）释出，提高谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活性，降低Ｉｔｏ 细胞ＨＡ、ＦＮ分泌诸方面优于ＶＥ或Ｓｅ 的单独作用，并可使Ｓｅ 的用量节省一半。结论：　ＶＥ和Ｓｅ具有抑制大鼠肝细胞、Ｉｔｏ 细胞ＥＣＭ的作用；二者的联合应用效果更好，可望成为一种抗肝纤维化的新方法。

EFFECTS OF VITAMIN E AND SELENIUM ON HEPATOCYTES AND ITO CELLS IN RATS

To study the effects of VE and Se on extracellular matrix (ECM) production of hepatocytes and Ito cells in vitro. Methods: Rat hepatocytes and Ito cells were isolated and cultured. Results: The results showed that the combination of VE and Se had better effects than VE or Se alone in inhibiting the incorporation of 3H proline and decreasing the release of malondialdehyde and increasing the activity of glutathione peroxidase in both hepatocytes and Ito cells. VE and Se jointly also had better effects than VE or Se alone in decreasing the secretion of hyaluronic acid、 fibronectin of Ito cells and decreasing the secretion of precollagen type Ⅲ of hepatocytes. The optimal concentration of VE and Se were 200 nmol/L and 0.36 nmol/L respectively. The combination of VE and Se not only saved the amounts of Se but also increased the inhibitory effects on ECMs production. Conclusion: Our studies suggest that the combination of VE and Se perhaps is one of main approaches in anti fibrosis therapy.