骨关节结核发病机制的相关实验研究

目的研究骨关节结核的发病机制。方法采用不同浓度结核分枝杆菌超声裂解产物体外诱导破骨细胞的生长;通过体外颅骨吸收实验,观察不同浓度结核分枝杆菌超声裂解产物诱导破骨细胞生成和钙离子释放的关系;体内注射结核分枝杆菌超声裂解产物,从血钙浓度和组织学方面观察骨质变化。结果结核分枝杆菌超声裂解产物能够在体外诱导破骨细胞生长,且破骨细胞数量的增加依赖细菌浓度的提高。钙离子释放与细菌诱导破骨细胞的数量相关,体内注射细菌能够引起破骨细胞数量增加、钙离子释放和骨质吸收。结论骨、关节结核的发病很可能与结核菌诱导破骨细胞增生有关。

Experimental study on the pathogenesis of osteoarticular tuberculosis

Objective To explore the pathogenesis of osteoarticular tuberculosis.Methods Mycobac-terial(Mt)sonicate in various concentrations was used to induce osteoclasts differentiation in vitro.The relationship between Mt sonicate-induced-osteoclasts and calcium releasing was observed in calvaria bone resorption test in vitro.Bone resorption in vivo as a result of local injection of Mt sonitate was observed.Results Mt sonicate induced the differentiation and proliferation of osteoclasts and Mt sonicates added to the media produced a dose-dependent stimulation of osteoclasts activity.Calcium releasing was correlated with the numbers of Mt sonicate-induced-osteoclasts.Local injection of Mt sonicate resulted in osteoclast proliferation,calcium releasing and bone resorption.Conclusions The pathogenesis of osteoarticular tuberculosis may well lie in osteoclasts proliferation and bone resorption induced by mycobacterium.