高糖条件下糖康乐对RPE细胞GSH表达的影响

目的探讨在高糖条件下糖康乐对兔视网膜色素上皮（RPE）细胞谷胱甘肽（GSH）表达的影响。方法采用体外培养的兔RPE细胞，分成空白对照组、高糖组、糖康乐组和牛磺酸组4个实验组（n=6），在高糖条件下糖康乐组加以不同质量浓度的糖康乐，对RPE细胞的GSH含量进行测定。结果RPE细胞的GSH含量在高糖作用后明显降低（t=5．72，P〈0．01），糖康乐在质量浓度为2μg／mL时可显著抑制GSH含量的降低（t=4．63，P〈0．01），在2p．g／mL时其作用优于阳性对照组的牛磺酸（t=4．74，P〈0．01）。结论一定质量浓度的糖康乐可以抑制高糖所导致的RPE细胞GSH含量的降低。

Effects of Tangkangle on production of glutathione by cultured retinal pigment epithelial cells in hyperglycemia condition

Objective Glutathione（GSH） is an important factor of anti-oxidation in retina. It has been demonstrated that exogenous GSH is a main way to maintain the GSH in retinal pigment epithelial（RPE） cells. However,less research on promoting GSH production in RPE cells using the compound derived from algae extractsis was seen. Present study was to investigate the effect of Tangkangle,on GSH production in cultured rabbit RPE cells under the hyperglycemia condition. Methods Rabbit RPE cells were isolated by trypsin digest and cultured in DMEM/F12 medium containing 10% fetal bovine serum and identified by ABC method. The 3rd generation of cells were divided into blank control group （without glucose） , high glucose （25 retool/L, HG） group, different concentrations of Tangkangle （0. 002, 0.02, 0.2, 2, 20, 200μg/mL） ＋ HG group and 1 mmol/L aminoethylsulfonic acid ＋ HG group （ positive control） according to the addition of high concentration of glucose and different concentrations of Tangkangle in medium. GSH production in the RPE cells was analyzed by detection of absorption of light at 405 nm. Results The GSH level in blank control group was 121.35 ＋ 5.44,and that in hyperglycemia condition was 44.77 ＋ 21.22 ,showing a remarkable reduction（ t =0.72, P 〈 0. 01 ）. The absorption values of RPE cells for GSH in 0. 2 - 20μg/mL Tangkangle ＋ HG groups and positive control group were significantly increased in comparison with HG group（ P 〈 0. 01 ） with a peak in 2 μg/mL Tangkangle ＋ HG group（ t = 4.63,P 〈 0. O1 ）. Conclusion Tangkangle retrieve GSH from the reduction by HG and can protect RPE cells from oxidative damage by HG.