炎性因子对C2C12细胞、MC-3T3-E1细胞成骨、成肌相关基因表达的影响

目的：探讨炎性因子对成肌和成骨细胞成肌、成骨相关基因表达的影响。方法：小鼠腹腔巨噬细胞上清液及白介素（IL）1β处理C2C12细胞及MC-3T3-E1细胞，观察炎性因子对两种细胞成骨、成肌相关基因的表达的影响。结果：小鼠腹腔巨噬细胞上清液促进C2C12细胞MyoD、Myogenin的表达，差别均有统计学意义（P〈0．001）；促进MC-3T3-E1细胞碱性磷酸酶（ALP）的表达，吸光度比值上升为原来的3倍多，差别有统计学意义（P〈0．001）。IL-1β单独对C2C12细胞和MC-3T3-E1细胞的成肌、成骨因子的表达影响无统计学意义（P〉0．05）。结论：巨噬细胞上清液可上调MC-3T3-E1细胞的成骨相关基因表达；上调C2C12细胞的成肌相关基因表达。IL-1β单独不能对C2C12细胞和MC-3T3-E1细胞的成肌、成骨基因的表达产生影响。

Effect of Inflammatory Factors on the Ossification and Myogenesis Related Gene Expressions in C2C12 and MC-3T3-E1 Cells

Objective： To explore the effect of inflammatory factors on the ossification and myogenesis related gene expressions in myoblasts and osteoblasts. Methods： C2C12 and MC-3T3-E1 cells were treated with supernatant of mice peritoneal maerophage and IL-1β. The ossification and myogenesis related gene expression in C2C12 and MC-3T3-E1 cells were examined. Results： Supernatant of mice peritoneal maerophage significantly up-regulated the MyoD and Myogenin expressions in C2C12 cells（P〈0.001） and ALP in MC-3T3-E1 cells （P〈0.001）. IL-1β alone had no effect on the gene expression of ossification and myogenesis in C2C12 and MC-3T3-E1 ceils （P〉0.05）. Conclusion： Supernatant of mice peritoneal macrophage could up-regulate myogenesis expression in C2C12 cells and ossification expression in MC-3T3-E 1 cells;IL-1 alone had no effect on the gene expression of ossification and myogenesis in C2C12 and MC-3T3-E1 ceils.