分析乳腺癌组织中ER、PR、PCNA及C-erbB-2表达的意义

目的 为了给临床选择乳腺癌术后辅助治疗方案提供参考依据,探讨乳腺癌预后因素的判断指标。方法 对198例乳腺癌组织蜡块,采用SABC法标记PCNA、C-erbB-2,亲和组化法标记ER、PR,分析其与年龄、病理类型、发病时月经状况、肿瘤大小及淋巴结转移数诸因素的相关性。结果 PCNA的表达与病理类型、淋巴结转移数及发病时月经状况有显著相关(P<0.05);C-erbB-2的表达亦与淋巴结转移及月经状况有显著相关(P<0.05)。ER、PR的表达均与月经状况显著相关(ER:P=0.000,PR:P=0.004)。C-erbB-2高表达与ER呈负相关(r=0.2337,P=0.001)。结论 PCNA高表达预后不良的标记,C-erbB-2高表达可为辅助治疗方案的选择提供依据。

The significance of evaluating the expressions of ER, PR, PCNA and C-erbB-2 on breast cancer

Objective In order to provide the objective evidences for the patient after breast cancer surgery in planning the therapeutic program and predicting the prognosis. Methods The expression of PCNA and C-erbB-2 were detected by SABC method. ER and PR were examined by affinity- histochemistry method with paraffin-embedded sections for 198 cases. The correlation analysis among the markers and the ages, pathological types, status of menstruation, sizes of tumor and lymph-node metastasis were done. Results The results showed that the expression of PCNA was significantly correlated with the pathological type of cancer, and the lymph-node metastasis and the status of menstruation (P<0. 05). The expression of C-erbB-2 was significantly correlated with the lymph-node metastasis and the status of menstruation (P<0. 05) as well. The expressions of ER and PR were significantly correlated with the status of menstruation. (ER: P = 0. 000, PR:F = 0. 004); The over-expression of C-erbB-2 was negatively correlated with the expression of ER (r = -0. 2337, P = 0. 001). Conclusions The over-expression of PCNA is a marker showing the poor prognosis, and the over-experssion of C-erbB-2 can be the evidence for programming the sequential therapy.