| 大鼠骨髓主要细胞成分的分离获取及其免疫调节作用 |
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| 引用本文： | 姜隽,欧阳芳,姜睿.大鼠骨髓主要细胞成分的分离获取及其免疫调节作用[J].泸州医学院学报,2008,31(2):121-125. |
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| 作者姓名： | 姜隽 欧阳芳 姜睿 |
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| 作者单位： | 1. 泸州医学院附属医院外科,四川泸州,646000
2. 泸州医学院附属医院内分泌科,四川泸州,646000 |
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| 摘 要： | 目的:建立分离获取大鼠骨髓主要细胞成分的方法,了解其免疫调节作用,为骨髓主要细胞成分输注诱导同种异基因移植物免疫耐受奠定基础。方法:采用密度梯度离心法、红细胞裂解法及细胞培养等方法,分离纯化供体大鼠骨髓细胞(donor bone marrow cells,DBMC)、骨髓间充质干细胞(mesenchymal stem cells,MSC)、不成熟树突细胞(immature dendritic cells,imDC),经光镜、电镜形态学、FCM测定imDC表面OX-62、MHC-Ⅱ、CD80和CD86表达、MSC表面CD80-CD86-和CD29表达等细胞表面标志的表型鉴定后,用MTT法检测DBMC、MSC和imDC对供受体混合淋巴细胞反应(Mixed lymphocytesreaction,MLR)的免疫调节作用。结果:分离获取大鼠骨髓主要细胞成分纯度可达85%-90%,活力>95%,均经光镜、电镜形态学、FCM等表型鉴定合格,其中,imDC表面OX-62、MHC-Ⅱ、CD80和CD86表达量分别是90.5%±16.4%、89.5%±6.2%、32.5%±9.2%和27.6%±8.3%;MSC表面CD80-C...
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| 关 键 词： | 大鼠 骨髓细胞 骨髓间充质干细胞 不成熟树突细胞 |
| 文章编号： | 1000-2669(2008)2-0121-05 |
| 修稿时间： | 2007年10月30 |
ISOLATING AND PURIFYING MAIN CELLS FROM RAT BONE MARROW |
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| Jiang Jun,et al.ISOLATING AND PURIFYING MAIN CELLS FROM RAT BONE MARROW[J].Journal of Luzhou Medical College,2008,31(2):121-125. |
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| Authors: | Jiang Jun |
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| Institution: | Jiang Jun,et al Department of Surgery,the Affiliated Hospital of Luzhou Medical College |
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| Abstract: | Objective:To isolate donor rat bone marrow cells(DBMC), mesenchymal stem cells(MSC) and immature dendritic cells(imDC) from the rat bone marrow an analyze their immunologic regulatory effects.Methods:The rat DBMC, MSC and imDC from rat femora marrow cells were separated and purified by gradient centrifugation,cell culture,etc. The morphological character of the cultured MSC and imDC was observed under light microscope and electron microscope. Expression of CD molecules were detected by flow cytometry. The i... |
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| Keywords: | Rat Bone marrow Mesenchymal stem cells imDC |
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