| 基于UPLC/LTQ-Orbitrap-MS的黄芩中黄酮碳苷的结构表征及同分异构体的区分 |
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| 引用本文： | 谢彤,徐建亚,沈存思,狄留庆,汪受传,单进军.基于UPLC/LTQ-Orbitrap-MS的黄芩中黄酮碳苷的结构表征及同分异构体的区分[J].中草药,2015,46(22):3318-3325. |
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| 作者姓名： | 谢彤 徐建亚 沈存思 狄留庆 汪受传 单进军 |
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| 作者单位： | 南京中医药大学中医儿科学研究所, 江苏省儿童呼吸疾病(中医药)重点实验室, 江苏 南京 210023;南京中医药大学中医儿科学研究所, 江苏省儿童呼吸疾病(中医药)重点实验室, 江苏 南京 210023;南京中医药大学中医儿科学研究所, 江苏省儿童呼吸疾病(中医药)重点实验室, 江苏 南京 210023;南京中医药大学药学院, 江苏省中药高效给药系统工程技术研究中心, 江苏 南京 210023;南京中医药大学中医儿科学研究所, 江苏省儿童呼吸疾病(中医药)重点实验室, 江苏 南京 210023;南京中医药大学中医儿科学研究所, 江苏省儿童呼吸疾病(中医药)重点实验室, 江苏 南京 210023;南京中医药大学药学院, 江苏省中药高效给药系统工程技术研究中心, 江苏 南京 210023 |
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| 基金项目： | 国家自然科学基金青年基金(81303295);国家自然科学基金面上项目(81373688);江苏省高校自然科学研究面上项目(13KJB360005) |
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| 摘 要： | 目的建立黄芩Scutellaria baicalensis的UPLC/LTQ-Orbitrap-MS分析测定方法,研究黄芩中黄酮碳苷类成分,并区分其中的同分异构体。方法采集Orbitrap全扫描的质谱图,并触发动态数据依赖分析模式采集二级、三级和四级的碎片信息,通过图谱解析确定黄酮碳苷的结果,通过比对碎片信息区分同分异构体。结果在黄芩中共鉴定表征了19个黄酮碳苷类成分,并对其中的同分异构体进行区分。结论该方法能够准确地筛选黄芩中的黄酮碳苷类成分,并对同分异构体进行区分,为其他中药的成分分析提供参考。
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| 关 键 词： | 黄芩 黄酮碳苷 UPLC/LTQ-Orbitrap-MS 同分异构体 结构表征 |
| 收稿时间： | 2015/5/17 0:00:00 |
Structural characterization of C-glycosyl flavonoids in Scutellaria baicalensis and differentiation of isomers by UPLC/LTQ-Orbitrap-MS |
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| XIE Tong,XU Jian-y,SHEN Cun-si,DI Liu-qing,WANG Shou-chuan and SHAN Jin-jun.Structural characterization of C-glycosyl flavonoids in Scutellaria baicalensis and differentiation of isomers by UPLC/LTQ-Orbitrap-MS[J].Chinese Traditional and Herbal Drugs,2015,46(22):3318-3325. |
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| Authors: | XIE Tong XU Jian-y SHEN Cun-si DI Liu-qing WANG Shou-chuan and SHAN Jin-jun |
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| Institution: | Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing 210023, China;Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing 210023, China;Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing 210023, China;Jiangsu Provincial TCM Engineering Technology Research Center of High Efficient Drug Delivery System, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China;Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing 210023, China;Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, Nanjing University of Chinese Medicine, Nanjing 210023, China;Jiangsu Provincial TCM Engineering Technology Research Center of High Efficient Drug Delivery System, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China |
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| Abstract: | Objective An UPLC-LTQ-Orbitrap-MS spectrometry method has been developed for the efficient separation and detection of C-glycosyl flavonoids in the extract of Scutellaria baicalensis differentiation of isomers. Methods Data were acquired by Orbitrap. MS2, MS3, and MS4 data were triggered by data-dependent acquisition mode. Isomers of C-glycosyl flavonoids were distinguished by a comparison study of mass distribution. Results A total of 19 compounds were identified as C-glycosyl flavonoids, and several isomers were successfully discriminated. Conclusion This method is proved to be powerful for the identification of C-glycosyl flavonoids and provides the important complementary information for other flavonoid analysis. |
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| Keywords: | Scutellaria baicalensis Georgi C-glycosyl flavonoids UPLC/LTQ-Orbitrap-MS isomers structural characterization |
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