ACE2基因多态性与心肌梗死后心功能不全及心室重构的相关性研究

目的研究血管紧张素转换酶2基因A9570G多态性与心肌梗死后心功能不全及心室重构的关系。方法收集252例陈旧性心肌梗死患者，采取外周血2ml提取DNA，多聚酶链扩增反应及限制性内切酶法检测ACE2基因A9570G基因型，按基因型进行分组，采用超声心动图比较不同基因型间患者LVEF，E／A，LVEDd及LVMI的差异。结果在男性，G基因型组LVEF、LVEDd、LVMI与A基因型组比较，差异有统计学意义（t=2．609、3．527、2．063，P〈0．05），而E／A两组比较，差异无统计学意义（P〉0．05）；在女性，3种基因型间患者LVEF、E／A、LVEDd及LVMI差异均无统计学意义（P〉0．05）。结论ACE2基因A9570G多态性与男性心肌梗死后心室重构及心功能不全相关，ACE2基因A9570G多态性可能是影响心梗后心室重构及心功能不全的遗传因素。

Association between the polymorphism of A9570G in angiotensin I converting enzyme 2 gene and cardiac dysfunction and ventricular remodeling after myocardial infarction

Objective To determine the effects of polymorphism of A9570G in angiotensin I converting enzyme 2 gene ( ACE2) gene on cardiac dysfunction and ventricular remodeling after myocardial infarction. Methods 252 patients with old myccardial infarction were included in this study. They were classified according to their polymorphisms of ACE 2 gene analyzed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Echocardiograms were used to determine left ventricular end diastolic diameters (LVEDd) , Left ventricular mass index (LVMI) , mitral flow pattern early diastolic and late diastolic peak flow ratio (E/A) and left ventricular ejection fraction ( LVEF). Results In male, LVEDd, LVMI, and LVEF had significant difference among ACE2 genotypes ( t = 2. 609,3.527and 2.063, P =0.010,0.001 and 0.041), and no significant differences in E/A( t =0.689,P =0.492). In female,LVEDd, LVMI, E/A and LVEF had no significant difference among ACE2 genotypes( F =0. 848, 0.077,0. 985 and 1.611, P = 0. 432,0. 926,0. 377 and 0. 205 ). Conclusion The polymorphism of A9570G in ACE2 gene may be associated with cardiac dysfunction and ventricular remodeling after myocardial infarction in male. ACE 2 gene polymorphism may be a genetic factor on cardiac dysfunction and ventricular remodeling after myocardial infarction.