| A549细胞系中自噬泡与线粒体形态的初步定量研究 |
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| 引用本文： | 任雅丽,李雪迎,王书合,柴立军,谢燕玲,程明. A549细胞系中自噬泡与线粒体形态的初步定量研究[J]. 中国体视学与图像分析, 2011, 0(2): 193-197 |
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| 作者姓名： | 任雅丽 李雪迎 王书合 柴立军 谢燕玲 程明 |
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| 作者单位： | 北京大学第一医院电镜室;北京大学第一医院统计室; |
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| 摘 要： | 目的探讨发生自噬的细胞中自噬泡的特点及自噬泡与细胞中线粒体形态特征参数间的关系,分析自噬泡膜来源于线粒体膜的可能性。方法将A549细胞培养后收集1～5×106个,常规固定、包埋及超薄切片,在透射电镜下观察。由随机取样的原则选择细胞进行照相。应用CMIAS多功能真彩色图像分析系统测定细胞、线粒体及自噬泡等结构的截面积和截面数目等参数,根据自动测量结果将细胞分为3组。结果 3组细胞内自噬泡总截面面积的平均值明显不同;3组间自噬泡体积密度的平均值之间存在统计学差异(P=0.03)。3组细胞内线粒体数目及线粒体总截面面积的平均值呈相应下降。结论由于3组细胞内线粒体和自噬泡总截面面积的平均值变化趋势相反,推测部分自噬泡的膜可能来源于线粒体的膜。
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| 关 键 词： | 体视学 电镜 自噬 线粒体 非小细胞肺癌 |
Preliminary morphometric analysis on the correlation between autophagosomes and mitochondria in non-small cell lung cancer A549 cells |
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| REN Yali,LI Xueying,WANG Shuhe,CHAI Lijun,XIE Yanling,CHENG Ming. Preliminary morphometric analysis on the correlation between autophagosomes and mitochondria in non-small cell lung cancer A549 cells[J]. Chinese Journal of Stereology and Image Analysis, 2011, 0(2): 193-197 |
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| Authors: | REN Yali LI Xueying WANG Shuhe CHAI Lijun XIE Yanling CHENG Ming |
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| Affiliation: | REN Yali1,LI Xueying2,WANG Shuhe1,CHAI Lijun1,XIE Yanling1,CHENG Ming1(1.Department of Electron Microscopy,Peking University First Hospital,Beijing 100034,China,2.Department of Biostatistics,China) |
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| Abstract: | Objective To assess the characteristics of autophagosomes in non-small cell lung cancer A549 cells and to examine the correlation of the related morphological parameters between autophagosomes and mitochondria aiming to assess the possibility that part of the autophagosomic membrane derives from mitochondrial membrane.Methods Images of A549 cells were observed and obtained by the electron microscopy.The areas and number of the cells,autophagosomes and mitochondria were analyzed by CMIAS.The cells were divid... |
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| Keywords: | morphometry electron microscopy autophagy mitochondria lung cancer cells |
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