癫痫大鼠海马区增殖的内源性神经前体细胞演变过程的研究

目的 追踪观察匹罗卡品诱导癫痫模型大鼠海马区增殖的内源性神经前体细胞的分化及演变过程，从而探讨痫性发作对增殖的内源性神经前体细胞的影响。方法 氯化锂和匹罗卡品联合诱导大鼠癫痫模型，正常对照组注射生理盐水，干预后14d腹腔注射5-溴脱氧尿苷嘧啶（BrdU）标记海马DG区增殖的内源性神经前体细胞；用免疫组化方法分别观察标记后5、14、28d海马DG区BrdU／神经元核性蛋白（NeuN）、BrdU／胶质纤维酸性蛋白（GFAP）表达；TUNEL标记方法观察BrdU／TUNEL的表达。结果 与正常对照组相比，癫痫模型组大鼠海马区出现的BrdU阳性细胞明显增多，增殖的细胞大多数分化为神经元（约70％），只有少数分化为星形胶质细胞。随标记时间延长，存活的增殖细胞逐渐减少，部分BrdU阳性细胞表现为TUNEL阳性。结论 癫痫发生后出现了神经前体细胞的增殖，增殖的细胞大多数分化为神经元。但只有少部分细胞存活下来参与海马结构的重组。

Fate of Newborn Inherent Neural Progenitor Cells in the Hippocampus after Pilocarpine-induced Status Epilepticus in Adult Rats

Objective To observe the fate of the newborn inherent neural progenitor cells(NPCs) after lithium-pilocarpine-induced status epilepsy(SE) in adult rats.Methods The adult rats were injected with lithium and pilocarpine to induce SE and normal saline was injected into the controls.Fourteen days after intervention,bromodeoxyuridine(BrdU) was injected intraperitoneally to label the newborn inherent NPCs in hippocampus DC region.By using immunohistochemistry,the expression of NeuN and GFAP in the BrdU positive cells was detected in hippocampal DG region 5,14 and 28 days after labeling respectively.The apoptosis of BrdU positive cells was assayed by using TUNEL.Results Compared with the control group,BrdU positive cells in hippocampus were significantly increased in SE model group.Most of newborn NPCs differentiated into mature neurons(about 70 %) and few into astrocytes.With labeled time prolonged,the number of BrdU positive cells was gradually decreased.Part of BrdU positive cells were positive for TUNEL staining.Conclusion SE stimulates the proliferation of newborn inherent NPCs in the hippocampus,and most of them differentiate into neurons.Only little newborn inherent NPCs survived and took part in the reconstruction of the hippocampal structure.