骨形成蛋白-7对人肾小管上皮细胞株HK-2凋亡的影响

研究不同浓度的骨成形蛋白 7(BMP 7)对人近曲小管上皮细胞株HK 2凋亡的影响并初步探讨其可能的机制。应用Hoechst 332 5 8荧光染色、DNA电泳检测凋亡细胞的形态学和生化改变 ,流式细胞仪定量检测细胞的凋亡率 ,并观察凋亡特异性蛋白酶Caspase 3活性变化的情况。结果显示 ,HK 2细胞加入 1 0ng/mlTGF β处理 2 4h后 ,可明显抑制细胞生长并诱导细胞凋亡 ,呈现明显的凋亡形态学改变 ,胞核或核质内可见浓度致密的颗粒状荧光 ,DNA电泳呈现典型的“梯形条带” ,而预先加入 5 0ng/ml、 1 0 0ng/mlBMP 7共处理 2 4h后 ,可减轻TGF β对细胞的抑制作用 ,凋亡细胞亦减少 ,Caspase 3蛋白酶活性明显下降 ,细胞凋亡率下降。提示BMP 7对人肾小管上皮细胞凋亡的具有抑制作用 ,可能是通过抑制Caspase蛋白酶活性而实现的

Effect of Bone Morphogenetic Protein-7 on Apoptosis of Renal Epithelial Cells Induced by TGF-β

To study the effect of bone morphogenetic protein 7 (BMP 7) on apoptosis of human renal epithelial cells induced by TGF β,morphological and biochemical evaluation of apoptosis were performed by Hoechst 33258 staining and DNA electrophoresis,and apoptosis rate were measured by flow cytometry,as well as the activity of Caspase 3 were evaluated After incubation of HK 2 cells with 10 ng/ml TGF β for 24 hours,the growth of cells were significantly inhibited and the apoptosis of cells were induced The HK 2 cells were treated with 50 ng/ml,100 ng/ml BMP 7 for 24 hours,and the apoptosis rate and the activity of Caspase 3 were obviously decreased Our results suggest that TGF β can induce apoptosis of human renal epithelial cells and BMP 7 play an anti apoptotic role on human renal epithelial cells,mainly by inhibiting the activity of Caspase 3