参附注射液对小鼠移植心脏细胞粘附分子表达强度的影响

目的通过检测小鼠移植心脏的细胞粘附分子，探讨参附注射液对移植心脏缺血再灌注损伤的保护作用。方法40只同基因近交系Balb／c小鼠，随机分为参附组与对照组，每组雌雄各10只，进行相同性别间配对移植，参附组于移植前连续3d每24h经腹腔给予参附注射液，最后1次给药为移植前30min；而对照组同法给予等量0，9％氯化钠注射液。分别于移植后4、24、72h及1周时对受体及移植心脏的成活率及移植物的形态进行观察，并通过免疫组织化学方法进行细胞内粘附分子-1（ICAM—1）、血管细胞粘附分子-1（VCAM—1）、血小板内皮细胞粘附分子（PECAM）、淋巴细胞功能相关抗原-1（LFA-1）染色，比较两组上述粘附分子的表达强度及LFA-1阳性细胞率的差别。结果参附组ICAM-1、VCAM-1的表达强度在移植后各时间点明显低于对照组（均P〈0．05）；PECAM的表达强度未见明显差别；LFA-1阳性细胞率均低于对照组（P〈0．05）。参附组内各时间点比较，ICAM-1、VCAM-1、PECAM的表达强度和LFA-1阳性细胞率均在24h最高。结论参附注射液可有效抑制小鼠心脏移植后移植物内ICAM-1、VCAM-1的表达，减少LFA-1阳性细胞的浸润。

The expression of adhesion molecules in murine cardiac grafts after administration of Shenfu injection

Objective To investigate the protective effects of Shenfu injection against ischemic-reperfusion (IR) injury in murine cardiac transplantation. Methods Cardiac transplantations were performed between MHC fully-matched inbred mice. The animals were given Shenfu infection (Shenfu group) or normal saline (control group). Heart grafts were harvested at 4hr, 24hr, 72hr and 1 week after transplantation. H.E. staining and immunohistochemistry were performed to evaluate the morphology of the grafts and the expression of ICAM-1, PECAM, VCAM-1, LFA-1 molecules. Results In Shenfu group the expression of ICAM-1 and VCAM-1 was weaker than that of control group, particularly in the early stage after IR. No difference was found in PECAM expression at any time point between 2 groups. In Shenfu group, the rate of LFA-1 positive cells was less than that of control group and most significantly at 24 hrs. Conclusion Shenfu Injection can inhibit the expression of ICAM-1 and VCAM-1 and reduce the infiltration of LFA-1 positive cells in murine cardiac grafts