| 高效液相色谱柱后衍生法测定豚鼠鼻粘膜中组胺 |
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| 引用本文： | 黄新华 陈本美. 高效液相色谱柱后衍生法测定豚鼠鼻粘膜中组胺[J]. 湖南医科大学学报, 2000, 25(3): 294-296 |
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| 作者姓名： | 黄新华 陈本美 |
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| 作者单位： | [1]设备处 [2]分析测试中心 |
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| 摘 要： | 通过比较各种分析柱的分离效果，建立用离子交换柱分离，邻苯二甲苯柱后衍生荧光检测并分析豚鼠鼻粘膜组织中组胺的液相色谱法。并对流动相的离子强度和ＰＨ进行了优化，确定以４０ｍｍｏｌ．Ｌ＾－１ＰＨ５．５０的柠檬酸钠缓冲液作为流动相为最侍者
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| 关 键 词： | 组胺 鼻粘膜 豚鼠 高效液相色谱柱后衍生法 |
High-performance liquid chromatography determination of histamine in nasal mucosa of guinea pig after post-column derivatization] |
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| X H Huang,B M Chen,S X Liang,F L Deng. High-performance liquid chromatography determination of histamine in nasal mucosa of guinea pig after post-column derivatization][J]. Bulletin of Hunan Medical University, 2000, 25(3): 294-296 |
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| Authors: | X H Huang B M Chen S X Liang F L Deng |
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| Affiliation: | Department of Equipment Management, Hunan Medical University, Changsha 410078. Analytical and Testing Center, Hunan Medical University, Changsha 410078. |
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| Abstract: | A rapid and simple method for determining histamine by post-column derivatization liquid chromatography with fluorescent detection was described. SCX weakly acidic cation exchange column was used as an analytical column. Histamine was eluted at 13 min 12 s by 40 mmol.L-1 of trisodium citrate (pH5.50) at flow rate of 1.0 ml.min-1. The recoveries of histamine ranged from 1.0 mumol.L-1 to 100 mumol.L-1 were above 92%. The detection limit for histamine was 50 nmol.L-1 and the linear range for histamine was 50 nmol.L(-1)-500 mumol.L-1. |
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