RNA干扰技术抑制卵巢癌细胞系SKOV3.ip1细胞内Her-2/neu基因表达的研究

目的 探讨卵巢癌细胞株SKOV3．ip1细胞转染表达短发卡状RNA（shRNA）的质粒后，SKOV3．ip1细胞中Her-2／neu基因表达的变化，和对SKOV3．ip1细胞凋亡的影响。方法 将针对Her-2／neu基因的shRNA表达载体转染SKOV3．ip1细胞，从mRNA水平、蛋白表达水平和细胞凋亡的变化三个方面评价shRNA表达载体对Her-2／neu基因表达的抑制作用。结果shRNA表达载体可以有效的抑制SKOV3．ip1细胞Her-2／neu基因的表达，使Her-2／neu基因的mRNA和蛋白表达量明显下降，细胞凋亡增多。结论靶向Her-2／neu的shRNA表达载体可以有效抑制Her-2／neu基因的表达。

Inhibitory effects of Her- 2/neu gene in SKOV3.ip1 cell by RNA interference

Objective To study the inhibition of Her-2/neu gene in SKOV3.ip1 cell,a cell line of ovarian cancer, transfected with plasmid expressing short hairpin RNA(shRNA) targeting Her-2/neu gene. Methods After the SKOV3.ip1 cells were transfected with plasmid expressing shRNA,the expression of Her-2/neu gene was assessed in both mRNA and protein levels.Apoptosis of transfected cells were also detected to evaluate the biological effect of this method. Results RT-PCR and Western blot results indicated that the Her-2/neu gene exression was efficiently suppressed by plasmid expressing shRNA targeting Her-2/neu.Apoptosic rate of transfected cells were increased obviously. Conclusion The plasmid expressing shRNA targeting Her-2/neu can inhibit the expression of Her-2/neu gene effectively.