流体切应力对内皮细胞粘附分子表达的影响

在动脉粥样硬化(Atherosclerosis,As)的发生发展中各种白细胞，包括单核细胞对内皮细胞的粘附可能起着较为重要的作用。在体内，血流切应力对内皮细胞的形态和功能有重要影响。为了阐明流体切应力对内皮细胞表面粘附分子表达的影响，本文研究了流体切应力(2.23～6.08dyne/cm

Effect of Flow Shear Stress on the Expression of Adhesion Molecules of Endothelial Cells

Adherence of various leukocytes, including monocyte, to vascular endothelial cells may play an important role in the development of atherosclerosis. In vivo, the hemodynamic shear forces have a critical effect on the surface expression of adhesion proteins. In order to elucidate the effect of flow shear stress on the expression of adhesion molecules of endothelial cells, we investigated the effect of flow shear stress (2.23-6.08 dyne/cm2) on the expression of adhesive molecules, intercellular adhesion molecule(ICAM-1), vascular cell adhesion molecule (VCAM-1), and E-selectin on cultured human umbilical vein endothelial cells(HUVECs). The expression of adhesion molecules on the surface of HUVECs induced by shear stress was analyzed using flow cytometry. The results showed that compared with stationary control, the surface expression of ICAM-1 was significantly increased (P < 0.05) on HUVECs after exposure to different shear stress (2.23, 4.20, 6.08 dyne/cm2), and it was found to be in close relationship with the shearing time (r = 0.992, 0.997, 0.997; P < 0.05). After exposure to shear(2.23 dyne/cm2), VCAM-1 expression was significant increased(P < 0.05), and it was positively correlated with the shearing time(r = 0.930; P < 0.05), while VCAM-1 expression dropped down to basal level(P < 0.05) after it was sheared at 4.20 or 6.08 dyne/cm2, and the magnitude of the reduction of VCAM-1 expression was negatively correlated with the shearing time (r = -0.975, -0.989; P < 0.05). E-selectin expression was less sensitive to shear stress, especially at the lower magnitudes of shear. These results indicate that the increase of ICAM-1 and VCAM-1 expression in endothelial cells induced by low shear stress may play a prominent role in the development of both inflammation and atherosclerosis.