哮喘患儿血清IL 12 TGFβ1 与IgE 水平变化的研究

目的：检测哮喘患儿不同病期的白细胞介素12 ( IL-12) 、转化生长因子β1 ( TGFβ1 ) 与免疫球蛋白E( IgE) 水平变化的规律，并探讨不同病期IL-12，TGFβ1水平与IgE水平的相关性，据此阐述它们在哮喘中的临床意义。方法：采用ELISA 方法检测85例哮喘患儿及30例正常儿童的血清IL-12，TGFβ1与总IgE 水平。结果：哮喘组血清IL-12，TGFβ1水平明显低于对照组，而IgE 水平则哮喘组明显高于对照组，且发作期IL-12，TGFβ1 水平（28.42±10.73 ng/L，40.25±11.73 pg/mL）明显低于缓解期（40.42±15.26 ng/L，65.41±22.38 pg/mL）,差异有显著性 (P< 0. 01)，缓解期血清IL-12，TGFβ1 水平明显低于对照组（67.42±20.58 ng/L，178.54±90.56 pg/mL）,差异有显著性(P<0.01)，发作期血清IgE 水平（280.35±80.54 IU/mL）明显高于缓解期（145.67±51.25 IU/mL）, 差异有显著性(P< 0.01), 缓解期血清IgE 水平明显高于对照组（53.61±13.32 IU/mL）, 差异有显著性(P<0.01)，哮喘患儿血清IL-12，TGFβ1水平与IgE呈负相关(P< 0.01)。结论：哮喘患儿存在IL-12，TGFβ1及IgE 水平失衡，提示IL-12，TGFβ1 在哮喘的发病中起着重要作用，检测它们的水平可为哮喘的诊断及判断病情提供依据。

Serum levels of IL 12, TGFβ1 and IgE in children with asthma

OBJECTIVE: This study examined the changes of serum levels of interleukin 12 ( IL-12), transforming growth factor beta 1 (TGFbeta 1) and immunoglobulin E ( IgE) in children with asthma as well as the correlation of IL-12 and TGFbeta 1 with IgE in order to investigate their roles in asthma. METHODS: Serum levels of IL-12 , TGFbeta 1 and IgE were detected using ELISA in 85 asthmatic children at the acute and the remission stages. Thirty healthy children served as control group. RESULTS: Compared with the control group, serum IL-12 and TGFbeta 1 levels were significantly lower and serum IgE levels were significantly higher in the asthmatic group through the acute to the remission stages. Serum IL-12 and TGFbeta 1 levels (40.42+/-15.26 ng/L and 65.41+/-22.38 pg/mL) significantly increased in the asthmatic group at the remission stage compared with those at the acute stage (28.42+/-10.73 ng/L and 40.25+/-11.73 pg/mL) (P<0.01), but remained lower levels than those in the control group (67.42+/-20.58 ng/L and 178.54+/-90.56 pg/mL) (P<0.01). The asthmatic patients at the remission stage showed significantly decreased serum IgE levels (145.67 +/-51.25 IU/mL) compared with those at the acute stage (280.35 +/-80.54 IU/mL) (P<0.01), but the IgE level in the remission stage was obviously higher than in the control group (53.61+/-13.32 IU/mL) (P<0.01). Serum IL-12 and TGFbeta 1 levels were negatively correlated with serum IgE level in asthmatic children. CONCLUSIONS: There might be an imbalance in serum IL-12, TGFbeta 1 and IgE levels in asthmatic children. IL-12, TGFbeta 1 and IgE may play an important role in the pathogenesis of asthma. They may be useful in the diagnosis and severity evaluation of asthma.