纤维粘连蛋白对人涎腺腺样囊性癌细胞株侵袭和转移的影响

为了研究纤维粘连蛋白（FN）与人涎腺腺样囊性癌（Acc）转移的关系，本研究应用免疫组织化学染色，Boyden小室体外移动试验和体外粘附试验，对人涎腺腺样囊性癌细胞系ACC—2和从其克隆出的高转移细胞株ACC—M细胞进行比较研究。结果发现FN在ACC—M表达明显高于ACC—2。在加入外源性FN后，ACC—2体外移动性、粘附率均明显提高，已接近ACC—M，而ACC—M无明显变化。结果表明FN在Acc侵袭和转移中起重要的调节作用。

Effects of fibronectin on the invasive and metastatic behaviors of human salivary adenoid cystic carcinoma cells

FN distribution in ACC-2 and high lung-metastatic ACC-M cells was studied with immunohistochemical staining. There were less FN found in ACC-2,but much more FN were observed in the highly metastatic clone Acc-M.In vitro motility of two cell lines was measured by Boyden chamber. With the presence of exgenous FN,Acc-2 became activated with a higher migration rate. The cell adhesion test showed that Ace-2 had stronger ability of adhe-sion than Ace-M with the presence of FN. The result suggested FN may play a vitral role in the invasion and metastasis of ACC.