Intraspecies variation of Brugia spp. in cat reservoirs using complete ITS sequences |
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Authors: | Supatra Areekit Pirom Singhaphan Sintawee Khuchareontaworn Pornpimon Kanjanavas Thayat Sriyaphai Arda Pakpitchareon Paisan Khawsak and Kosum Chansiri |
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Institution: | (1) Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Sukhumvit 23, Bangkok, 10110, Thailand |
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Abstract: | The internal transcribed spacer (ITS) region was used to study the intraspecies variation of Brugia spp. in cat reservoirs. Blood specimens from seven naturally infected cats were collected from two different geographical
brugian-endemic areas in Thailand. The DNAPAR tree of these Brugia spp. was constructed using a maximum likelihood approach based on ITS nucleotide sequences and was compared to those of Brugia malayi, Brugia pahangi, and Dirofilaria immitis that were previously reported in GenBank. The phylogenetic trees inferred from ITS1, ITS2, and complete ITS sequences indicated
that B. malayi and B. pahangi were separated into two clades, and subgroups were generated within each clade. The data revealed that ITS2 sequences were
less informative than ITS1 for studying intraspecies variation of Brugia spp. Our results are primary data for intraspecies variation of B. malayi and B. pahangi in cat reservoirs. The information could be applicable for studying the molecular epidemiology and the dynamic nature of
the parasites.
GenBank accession numbers of Brugia malayi and Brugia pahangi complete ITS regions using in this study were EU373601-EU373625 and EU373626- EU373655, respectively. |
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